ID   A0A1M5BFZ5_9FIRM        Unreviewed;       462 AA.
AC   A0A1M5BFZ5;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=23S rRNA m(5)U-1939 methyltransferase {ECO:0000313|EMBL:SHF41376.1};
GN   ORFNames=SAMN02745133_02586 {ECO:0000313|EMBL:SHF41376.1};
OS   Desulforamulus putei DSM 12395.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC   Desulforamulus.
OX   NCBI_TaxID=1121429 {ECO:0000313|EMBL:SHF41376.1, ECO:0000313|Proteomes:UP000184148};
RN   [1] {ECO:0000313|EMBL:SHF41376.1, ECO:0000313|Proteomes:UP000184148}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12395 {ECO:0000313|EMBL:SHF41376.1,
RC   ECO:0000313|Proteomes:UP000184148};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FQUY01000022; SHF41376.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1M5BFZ5; -.
DR   STRING; 1121429.SAMN02745133_02586; -.
DR   OrthoDB; 9804590at2; -.
DR   Proteomes; UP000184148; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          4..62
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        414
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        414
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         289
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         318
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         339
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         387
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   462 AA;  51133 MW;  1D0A6D55FF89B037 CRC64;
     MEKVIKTGDL LDLTIQGLGH AGEGVGRYEN LAVFVPGVLP GERARVRITR VKKSFARGEA
     VDILEQAANR VVPPCPVSHR CGGCQLQHLE YQAQLDHKRA VVQAALTRLG GLEQVEVQPT
     LGMTDPWHYR NKIHLQVKQE GHRVKLGFYA EGTYQLATGP GHQTCLLVDK QINQVAATLE
     ELINEQAVPP YDWQKKSGLL RHVMIRRGFR TGEIMVVLVT SSQRWPAARE FARAIVTRHP
     EVVSVIRNIN TSPGRVVLGA ENRLLTGREY IFDYLKDLKF KISAASFYQV NPLQTEVRYN
     RVLQLADLKG NETVVDAYCG IGTIANFLAR HAGRVIGMEI VPEAVEDAKE NALLNGLSNT
     EFIPGPVESL LPNMVAQGLK PDLVVLDPPR KGCAKEVLQA ISKAQAPRVV YVSCDPGTLA
     RDLGILDASG YQTLLAQPVD MFPWTSHVEC IIQIKRAESR MK
//