ID A0A1M5BS81_9GAMM Unreviewed; 1149 AA.
AC A0A1M5BS81;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=SAMN02745148_02658 {ECO:0000313|EMBL:SHF45276.1};
OS Halomonas ilicicola DSM 19980.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=1121942 {ECO:0000313|EMBL:SHF45276.1, ECO:0000313|Proteomes:UP000184346};
RN [1] {ECO:0000313|EMBL:SHF45276.1, ECO:0000313|Proteomes:UP000184346}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19980 {ECO:0000313|EMBL:SHF45276.1,
RC ECO:0000313|Proteomes:UP000184346};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; FQUJ01000012; SHF45276.1; -; Genomic_DNA.
DR RefSeq; WP_072823669.1; NZ_FQUJ01000012.1.
DR AlphaFoldDB; A0A1M5BS81; -.
DR STRING; 1121942.SAMN02745148_02658; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000184346; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000184346}.
FT DOMAIN 615..776
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 797..953
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1149 AA; 127791 MW; FB3F708B233DD4E7 CRC64;
MPNFSPLAPP APQGTRETLY WQAPQGAAGA LALARLADSA PLLVVTPDTA AAQRLEGALR
FFASVPVLPF PDWETLPYDS FSPHQDIVSE RLRTLRRLQE AREGIVLVPI NTLMQRLPPS
DYIAGRVVTL SVGMTIDREG FRERLSRAGY RAVETVYEPG EYALRGALID LFPMGSEAPL
RIDLFDDEID TLRHFDPDTQ RSQDKVDSVE LLPAHEYSLS RSAIACFREG FETLFDVDPR
QCPLYNDALK GIPSPGLEQY LPLFFEETAT LFDHLAEGTR VALLPGAHEA AEHHWAAIQS
RYENLGVDPT RPLLPPARAF VPVPEVFAAI KAKPRVALVE DETHPHAHHP STQAPPQVAI
NARAQKPLAR LDEFLAAHGE LRVLFVAESR GRREALEETL AVLHLDMPHV GDWQAFLDSG
SRLAITESEL DEGLWLADPA LAVVTETELF GEVVRQTRRR EKATDDNELA VRHLSELRPG
APVVHQTHGV GRYQGLEALE AGGQAAEFVA LEYAGGAKLY VPVDNLHLIS RYAGASEELA
PLHRLGSDSW DKAKKKAAEK IRDTAAELLD VYARREAREG FACAEPGEEY QRFAASFPFE
ETPDQRAAIH AVIGDMVAPR PMDRVVCGDV GFGKTEVAMR AAFLAVHSER QVVVLVPTTL
LAQQHFDNFR DRFADTAVQI ELVSRFTGGK GQAESLKRIE DGRADIVIGT HKLLSKSMKF
PNMGLLIIDE EHRFGVAQKE RLKSLRAEVD ILTLTATPIP RTLNMAMSGM RDLSIIATPP
ARRLSVKTFV QQRNEPVIKE AILREILRGG QVYLLHNEVR TIEQTAEKVR ELVPEARVGV
AHGQLPERSL ERIMSDFYHK RFNVLVCSTI IETGIDVPSA NTILIERADK FGLAQLHQLR
GRVGRSHHQA YAYLLTPPPK AITKDALKRL EAIGQAEDLG AGFTLASHDM EIRGAGELLG
DEQSGQMETI GYSLYMQMLD RAVKAIRAGK TPNIEAPLDE GVEVGLNLPA LIPDDYLHDV
QQRLIMYKRI SNAENEADLR ELQVEMIDRF GLLPSPVKTL FRQTRLRQRA ERLGIIRLEA
GPERGRVIFG ANPAVDPMTL VDLIQRDPVH YRLDGADTLR FEIDMETEEA RFQAVEGLLE
RLNRKAQAA
//
