ID A0A1M5E7T9_9ACTN Unreviewed; 1274 AA.
AC A0A1M5E7T9;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=2-oxoglutarate dehydrogenase E1 component {ECO:0000313|EMBL:SHF75121.1};
GN ORFNames=SAMN05443575_0810 {ECO:0000313|EMBL:SHF75121.1};
OS Jatrophihabitans endophyticus.
OC Bacteria; Actinomycetota; Actinomycetes; Jatrophihabitantales;
OC Jatrophihabitantaceae; Jatrophihabitans.
OX NCBI_TaxID=1206085 {ECO:0000313|EMBL:SHF75121.1, ECO:0000313|Proteomes:UP000186132};
RN [1] {ECO:0000313|EMBL:SHF75121.1, ECO:0000313|Proteomes:UP000186132}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45627 {ECO:0000313|EMBL:SHF75121.1,
RC ECO:0000313|Proteomes:UP000186132};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + H(+) + N(6)-[(R)-lipoyl]-L-lysyl-
CC [dihydrolipoyllysine-residue succinyltransferase] = CO2 + N(6)-[(R)-
CC S(8)-succinyldihydrolipoyl]-L-lysyl-[dihydrolipoyllysine-residue
CC succinyltransferase]; Xref=Rhea:RHEA:12188, Rhea:RHEA-COMP:10483,
CC Rhea:RHEA-COMP:10484, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:83099, ChEBI:CHEBI:83120; EC=1.2.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00043700};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(6)-[(R)-dihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase
CC complex component E2] + succinyl-CoA = CoA + N(6)-[(R)-S(8)-
CC succinyldihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase complex
CC component E2]; Xref=Rhea:RHEA:15213, Rhea:RHEA-COMP:10581, Rhea:RHEA-
CC COMP:10582, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:83100,
CC ChEBI:CHEBI:83120; EC=2.3.1.61;
CC Evidence={ECO:0000256|ARBA:ARBA00043693};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; succinyl-
CC CoA from 2-oxoglutarate (dehydrogenase route): step 1/1.
CC {ECO:0000256|ARBA:ARBA00004813}.
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DR EMBL; FQVU01000001; SHF75121.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M5E7T9; -.
DR STRING; 1206085.SAMN05443575_0810; -.
DR UniPathway; UPA00223; UER00997.
DR Proteomes; UP000186132; Unassembled WGS sequence.
DR GO; GO:0004149; F:dihydrolipoyllysine-residue succinyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0004591; F:oxoglutarate dehydrogenase (succinyl-transferring) activity; IEA:UniProtKB-EC.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd02016; TPP_E1_OGDC_like; 1.
DR Gene3D; 3.40.50.12470; -; 1.
DR Gene3D; 3.40.50.970; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.50.11610; Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain; 1.
DR Gene3D; 1.10.287.1150; TPP helical domain; 1.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR032106; 2-oxogl_dehyd_N.
DR InterPro; IPR011603; 2oxoglutarate_DH_E1.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001017; DH_E1.
DR InterPro; IPR031717; KGD_C.
DR InterPro; IPR042179; KGD_C_sf.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR NCBIfam; TIGR00239; 2oxo_dh_E1; 1.
DR PANTHER; PTHR23152:SF4; 2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1; 1.
DR PANTHER; PTHR23152; 2-OXOGLUTARATE DEHYDROGENASE; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF16078; 2-oxogl_dehyd_N; 1.
DR Pfam; PF00676; E1_dh; 1.
DR Pfam; PF16870; OxoGdeHyase_C; 1.
DR Pfam; PF02779; Transket_pyr; 1.
DR PIRSF; PIRSF000157; Oxoglu_dh_E1; 1.
DR SMART; SM00861; Transket_pyr; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
PE 4: Predicted;
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000186132};
KW Thiamine pyrophosphate {ECO:0000256|ARBA:ARBA00023052};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 937..1130
FT /note="Transketolase-like pyrimidine-binding"
FT /evidence="ECO:0000259|SMART:SM00861"
FT REGION 48..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1274 AA; 138624 MW; 8865646F9CC58DAC CRC64;
MATDQSTDNP STRGDFGANE WLVEDMYERY LDDPHSVDAA WHDFFADYRP GTASAPGSSS
ANGTASANGA ARTGTADPSD TAESTETSDT EPGRSPSAGV PEQAAPARTS PAPTDSPAAA
PAAPASDQNR PTPAASTDEG KAPEAPAAEN AKAAQPAPSA PAPKVEGPGR APLRGAAARV
VTNMEQSLHV PTATSVRAVP AKLLFDNRVV INNHLRRARG GKVSFTHLIG YAVVKALGVH
PEMNNAFAEV DGKPMLVTPE HVNLGIAIDL AGKNGARSLV VASIKGAETL DFAAFWNAYE
DIIRRARNGK LTTDDFAGTT ISLTNPGTIG TNHSVPRLMQ GQGTIVGVGS MEYPAEFSGM
NPAALLDRAI SKIMTLTSTY DHRIIQGAQS GEFLKVVHEL LLSDAFYDEI FAALKIPYEP
VRWLVDRDFT HEGQIGKNAR VIELINAYRT NGHLMADTDP LEFTVRSHPD LDITRHGLTL
WDLDREFPVD GFAGEKLMTL RDILGVLRDA YCRRVGVEYM HITDPAQRRW LQERIEIQQG
QPSRDEQLHI LGRLNVAEAF ETFLQTKYVG QKRFSLEGGE TVIALLDRVL AEAADQDLDE
VVIGMPHRGR LNVLANIVGK PYAKIFNEFE GNIDPGTAQG SGDVKYHLGA DGTFTSPSGH
EIAVSLTANP SHLEAVNPVL EGIVRAKQDK LDKGDDGFTV LPVLMHGDAA FAGQGVVAET
LNVSQLRGYR TGGTVHVVVN NQVGFTTSPS SSRSSLYCTD IARMISAPIF HVNGDDPEAC
VRVAELAVQY RREFRKDVVI DMVCYRRRGH NEADNPSFTQ PMMYDIIDAK RSVRKLYTEA
LVGRGDITLT DAEAALKDFQ AQLEKVFVET RDASDKAAPQ HEIERETPSQ QVTTAIRAEV
IKRIAEAYAN PPEGFTLHPR LKPQIDRRVA MASSGKVDWA TAELFAFGSL VMDGRTVRLS
GQDSRRGTFT QRHATLIDRN TGEEYTPLKQ LGEDQAPFFP YDSLLSEYAA VGFEYGYSVA
REDALVCWEA QFGDFVDGAQ TVIDEFISAG EAKWGQRSAV TLLLPHGYEG QGPDHSSARP
ERFLQLCAEN NMTVAMCSSP ANYFHLLRRQ GLSPVRRPLI AMTPKSLLRL KAAVSELDDF
TTGTFAPVLP DLSADAGKVT RVLLCAGKIY YDLVAEREKS GRDDVAIVRV EQLYPLPAAE
IRAQLATYRD AEVVWVQEEP ANQGGWPFMA LNLPEHLEGR GLRLASRRAS ASPAVGSLSV
HEAQQREVVT TAFG
//
