ID A0A1M5FG25_9BACE Unreviewed; 439 AA.
AC A0A1M5FG25;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE SubName: Full=Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB {ECO:0000313|EMBL:SHF90101.1};
GN ORFNames=SAMN05444405_11675 {ECO:0000313|EMBL:SHF90101.1};
OS Bacteroides luti.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=1297750 {ECO:0000313|EMBL:SHF90101.1, ECO:0000313|Proteomes:UP000184509};
RN [1] {ECO:0000313|EMBL:SHF90101.1, ECO:0000313|Proteomes:UP000184509}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26991 {ECO:0000313|EMBL:SHF90101.1,
RC ECO:0000313|Proteomes:UP000184509};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; FQTV01000016; SHF90101.1; -; Genomic_DNA.
DR RefSeq; WP_073403356.1; NZ_FQTV01000016.1.
DR AlphaFoldDB; A0A1M5FG25; -.
DR STRING; 1297750.SAMN05444405_11675; -.
DR OrthoDB; 9805215at2; -.
DR Proteomes; UP000184509; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035596; F:methylthiotransferase activity; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR006467; MiaB-like_bact.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR01579; MiaB-like-C; 1.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR11918; RADICAL SAM PROTEINS; 1.
DR PANTHER; PTHR11918:SF45; THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01061; methylthiotransferase; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000184509};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SHF90101.1}.
FT DOMAIN 10..122
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 145..373
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 439 AA; 49301 MW; 8576AB528D8F54E7 CRC64;
MIDTSIFQDK TAVYYTLGCK LNFSETSTIG KTLKEAGVRT ARKGEKADIC VINTCSVTEV
ADKKCRQAIH RFRKQHPDAF IVVTGCYAQL KPDQVAEIEG VDVVLGAEQK KDLLKHLGDL
HKHDKGEAVA SSFKDIRSFA PSCSRGDRTR YFLKVQDGCD YFCSYCTIPL ARGRSRNGTI
ASMVEQAEQA AADGGKEIVL TGVNIGDFGR TTGESFFELV KALDKVQGIE RYRISSIEPN
LLTDEIIEYV SKSRSFMPHF HIPLQSGSDE VLKLMRRRYD TELFAGKIRK IKELMPDAFI
GVDVIVGTRG ETDEYFEKAY EFIQGLDVSQ LHVFSYSERP NTQALNIDYV VTPEVKHQRS
QRLLALSDEK TKAFYAKRIG ETMTVLMEKP KAGAPMHGFT DNYIRVEVES KPKLDNHLVK
VCLGEFNEDE TALKGTIIE
//
