ID A0A1M5FKR0_9THEO Unreviewed; 447 AA.
AC A0A1M5FKR0;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=23S rRNA (Uracil1939-C5)-methyltransferase {ECO:0000313|EMBL:SHF92075.1};
GN ORFNames=SAMN02746089_02774 {ECO:0000313|EMBL:SHF92075.1};
OS Caldanaerobius fijiensis DSM 17918.
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobius.
OX NCBI_TaxID=1121256 {ECO:0000313|EMBL:SHF92075.1, ECO:0000313|Proteomes:UP000184088};
RN [1] {ECO:0000313|EMBL:SHF92075.1, ECO:0000313|Proteomes:UP000184088}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17918 {ECO:0000313|EMBL:SHF92075.1,
RC ECO:0000313|Proteomes:UP000184088};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
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DR EMBL; FQVH01000068; SHF92075.1; -; Genomic_DNA.
DR RefSeq; WP_073346634.1; NZ_FQVH01000068.1.
DR AlphaFoldDB; A0A1M5FKR0; -.
DR STRING; 1121256.SAMN02746089_02774; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000184088; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000184088};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 4..62
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 397
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 397
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 272
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 301
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 322
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 370
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 447 AA; 50126 MW; 249AE0469932C2BA CRC64;
MKQPVVVGES YIIDIVDLSS EGLGVGKVNG FTVFTRGAVI GERVEARIFK VTKNYAVADA
VKIIISSPDR IEPECNAKNC GGCQLLHLSY EGQLRYKTNK VKNMLKRIGK IEAKVNDIIG
ADKPWHYRNK AEFAVMWVDD DPVIGFRAPK SHDVIPVERC YIQDTAVMDV VKAVREFIKK
FANHEILSII TKLSSFNGNI MVILETVDKD LSDSEYLIDY LKQIKGVVSI VQLFKCEDKR
KREKTKILYG VNKIIDKIKD LYFNITPLSF FQVNPEQTVK LYDKVLEYAD LSGNEVVVDA
YCGIGTISLY LARKAKEVYG IEVVEEAVEA AKENAAINHI ANANFIAGKA EDELNSLYKR
GIVADVVVVD PPRRGCDSRL LETLAAMKPK RIVYVSCDPG TLARDLRFLA DNGFDVIEVQ
PVDMFPQTYH VEVVTLLVRN TKGFSDL
//
