ID A0A1M5GRT1_9GAMM Unreviewed; 737 AA.
AC A0A1M5GRT1;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=SAMN04487965_3233 {ECO:0000313|EMBL:SHG06381.1};
OS Microbulbifer donghaiensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
OC Microbulbiferaceae; Microbulbifer.
OX NCBI_TaxID=494016 {ECO:0000313|EMBL:SHG06381.1, ECO:0000313|Proteomes:UP000184170};
RN [1] {ECO:0000313|EMBL:SHG06381.1, ECO:0000313|Proteomes:UP000184170}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.7063 {ECO:0000313|EMBL:SHG06381.1,
RC ECO:0000313|Proteomes:UP000184170};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FQVA01000006; SHG06381.1; -; Genomic_DNA.
DR RefSeq; WP_073277083.1; NZ_FQVA01000006.1.
DR AlphaFoldDB; A0A1M5GRT1; -.
DR STRING; 494016.SAMN04487965_3233; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000184170; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 4..291
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 292..567
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 25..32
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 737 AA; 84724 MW; 2D6748DC7EA193FB CRC64;
MHPPELTEEQ QAIADHAGGH AKIIAVAGSG KTTALLHYVR NRLDAGTAAE RMLVLMYNRS
AREDFDRRLR QLLDGPAPAV HTFHSLGYRL YQRMIANRHI APANLTPLPQ STVQLQIWKA
IEECAPPYEL EDIRARKQSE IEAAEYFIDY TKTILTGDLS AFQELKLGDE YLYFLKVFRT
FEDWRRSQNA VTYADLIYDP AILLSLEPGI AEQYGSYYAD ILVDEYQDIN EVQHFLLRVL
YGKSGNVIAI GDPDQTIYEW RGSKPDFLLK FFDGDFPPSN VYQLSRTFRY GHQLSLAANH
FIGNNRERAD IFCVSANGEQ PTEIELVHSE KETRWLVDYL RRCQKRGRPL DQIAVLVRLW
SVAAPIELAL LASNIPYRSG NRNTVLSRRE LRPLFWSLNI ASGRFAELPA KRREQGFYEW
LTAPHIRIPR KVLQPLCAQL AAYGGNWGKQ LLGLIPESLS KAQLKRLKQR AELLTEMEQW
RGLAGELLRR QLGELDYLTG IGEDSFTRQQ GEERQQTILA FCQYLDSLKQ SPAAALAHLQ
ELQRQHREGE QTEADAVQIT TMHQAKGLEW DQVVIPSLTQ HNMPYQPQRD FTTPASLESE
RRLMYVAMTR ARHKLYLLAP PALREAGAEQ QPSLFIDEMQ LPLCKLFADA LQQRPANISS
SVPISRQALR YLEACDYNPE ISGPRMPMRK PALGEGIRHR KLGYGRVIKW EDERLEILFS
DRKTRRFDWE KLAQHLM
//
