ID A0A1M5N4P4_9GAMM Unreviewed; 528 AA.
AC A0A1M5N4P4;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=SAMN04488068_1623 {ECO:0000313|EMBL:SHG84425.1};
OS Hydrocarboniphaga daqingensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales; Nevskiaceae;
OC Hydrocarboniphaga.
OX NCBI_TaxID=490188 {ECO:0000313|EMBL:SHG84425.1, ECO:0000313|Proteomes:UP000199758};
RN [1] {ECO:0000313|EMBL:SHG84425.1, ECO:0000313|Proteomes:UP000199758}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.7049 {ECO:0000313|EMBL:SHG84425.1,
RC ECO:0000313|Proteomes:UP000199758};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; FQWZ01000003; SHG84425.1; -; Genomic_DNA.
DR RefSeq; WP_072896335.1; NZ_FQWZ01000003.1.
DR AlphaFoldDB; A0A1M5N4P4; -.
DR STRING; 490188.SAMN04488068_1623; -.
DR OrthoDB; 5378341at2; -.
DR Proteomes; UP000199758; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000199758};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 26..528
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023106756"
FT DOMAIN 55..102
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 195..344
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 347..527
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 337
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 429
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 528 AA; 55762 MW; EDA662D23E2F22DA CRC64;
MQHHKLLVSL GLALSLLAVN NGAQAAPDLN AAPVKRALAL IAAQPKRING SGADTYAARD
WIVDRSGAEH VRFDRRYRGL PVLGGDFVVH QDASGRLASV SQTLTRPLNV DLKAAFTEAD
AIRVAEAAFT GTREGRAIVS KLIDARSTPA VLAYDVRLAG VTEDGTPTRR HWLVNAGTLA
LIDGWDEVQT AAATATGNSL LSGVIALTTD SQSSGGYVLR DPSRGGQATV DLRNTRLAVL
AKAYTDSDNV WGDFTNSDRA TVGVDAAYGA ALTWDYFLAQ FGRRGIADDG VGATSRVHYG
RNYVNAFWDN GCFCMTYGDG DGGSYLPLVA VDIAGHEMTH GVTSRTANLT YSGESGGLNE
AMSDIFGTLV EYAANNSIST PNYLIGERIY TANNGSPTPT QALRYMFKPS LDNKSPDCYV
GNIGNLDVHY SSGVANHFFY LLTQGAVSPP GFTLAPSALV CNGNTAIRPI SRDQAAQILY
RALTVYMTSS TGYAGARSTT LQAAADLYGT QSAAYTAVAA AWAAVGVN
//
