ID A0A1M5SF30_9BACT Unreviewed; 730 AA.
AC A0A1M5SF30;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN ORFNames=SAMN05720761_11355 {ECO:0000313|EMBL:SHH36513.1};
OS Fibrobacter sp. UWCM.
OC Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales; Fibrobacteraceae;
OC Fibrobacter.
OX NCBI_TaxID=1896208 {ECO:0000313|EMBL:SHH36513.1, ECO:0000313|Proteomes:UP000184289};
RN [1] {ECO:0000313|EMBL:SHH36513.1, ECO:0000313|Proteomes:UP000184289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UWCM {ECO:0000313|EMBL:SHH36513.1,
RC ECO:0000313|Proteomes:UP000184289};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC Evidence={ECO:0000256|PIRNR:PIRNR009407};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC {ECO:0000256|PIRSR:PIRSR009407-3};
CC -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC {ECO:0000256|PIRNR:PIRNR009407}.
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DR EMBL; FQWR01000013; SHH36513.1; -; Genomic_DNA.
DR RefSeq; WP_073114977.1; NZ_FQWR01000013.1.
DR AlphaFoldDB; A0A1M5SF30; -.
DR Proteomes; UP000184289; Unassembled WGS sequence.
DR GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR NCBIfam; TIGR00178; monomer_idh; 1.
DR PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR Pfam; PF03971; IDH; 1.
DR PIRSF; PIRSF009407; IDH_monmr; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|PIRNR:PIRNR009407};
KW Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW NADP {ECO:0000256|PIRNR:PIRNR009407};
KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR009407};
KW Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR009407}.
FT BINDING 124..131
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 137
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 340
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 537
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 538
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 542
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT SITE 247
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT SITE 410
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
SQ SEQUENCE 730 AA; 79655 MW; 198733C8DE96579D CRC64;
MNTKIYYTLT DESPFLATQS LLPIVRGFAK AADIDVKTKN ISLPGRILAA FGKASDDLDF
LGKLTLEPDA NIIKLPNISA SVPQLKAAIA ELQKNGFDVP DYPDAPANDE EKAIRAKYDK
VKGSAVNPVL RQGNSDRRAP KAVKNFARNN PHSNGNWNTS VKTHVASMQA DDFYGNEKSI
TMADADTFKI EFVNEAGEVT ELRAAKPLLK GEIIDATVMR MASLEKFIAN AMAEAKAKGL
LFSVHLKATM MKVSDPVLFG AFVRVFFKDV FTKYVDLFKE LGIDANNGLG DLYKRLEGNA
KEAEVKAAID AALAAGPDLA MVDSAKGVTN LHVPSDVIID ASMPAMIRNS GCMWNKEGKL
QETIACIPDR CYAGIYDETI EFCKQNGAFD PKTMGTVPNV GLMAQGAEEY GSHDKTFVAK
GKGVIRAVNS KGEVLLQQNV EAGDIFRMCQ AKDAPVRDWV KLAVTRARLS NTPAIFWLDP
ERAHDREIQK KVEAYLPKHD LNGLDIKIMS PRKAIVETMK RAKAGLDTIG VTGNVMRDYL
TDLFPILEVG TSAKMLSIVP LMAGGGLYET GAGGSAPKQV QQFLAENYLR WDSLGEYFAL
VPAFEQVALK DGNKKAKVLA DTLDEANGKI LEFNRTPARK IGELDNRGSH FYLALYWSEA
LAAQKDDAEL AKKFAPVADA LKAREAEIVA AFAAEQGKPA DIGGYYLPKA DLLKKWMRPV
AEFNAVIDAL
//
