ID   A0A1M5TUW5_9BACT        Unreviewed;       849 AA.
AC   A0A1M5TUW5;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   ORFNames=SAMN04488109_4289 {ECO:0000313|EMBL:SHH54562.1};
OS   Chryseolinea serpens.
OC   Bacteria; Bacteroidota; Cytophagia; Cytophagales; Fulvivirgaceae;
OC   Chryseolinea.
OX   NCBI_TaxID=947013 {ECO:0000313|EMBL:SHH54562.1, ECO:0000313|Proteomes:UP000184212};
RN   [1] {ECO:0000313|EMBL:SHH54562.1, ECO:0000313|Proteomes:UP000184212}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 24574 {ECO:0000313|EMBL:SHH54562.1,
RC   ECO:0000313|Proteomes:UP000184212};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|RuleBase:RU361166};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
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DR   EMBL; FQWQ01000003; SHH54562.1; -; Genomic_DNA.
DR   RefSeq; WP_073139580.1; NZ_FQWQ01000003.1.
DR   AlphaFoldDB; A0A1M5TUW5; -.
DR   STRING; 947013.SAMN04488109_4289; -.
DR   OrthoDB; 5936802at2; -.
DR   Proteomes; UP000184212; Unassembled WGS sequence.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd02850; E_set_Cellulase_N; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR004197; Cellulase_Ig-like.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF02927; CelD_N; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW   Glycosidase {ECO:0000256|PROSITE-ProRule:PRU10060,
KW   ECO:0000256|RuleBase:RU361166};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU10060,
KW   ECO:0000256|RuleBase:RU361166};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184212}.
FT   DOMAIN          285..367
FT                   /note="Cellulase Ig-like"
FT                   /evidence="ECO:0000259|Pfam:PF02927"
FT   DOMAIN          452..836
FT                   /note="Glycoside hydrolase family 9"
FT                   /evidence="ECO:0000259|Pfam:PF00759"
FT   ACT_SITE        819
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        828
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   849 AA;  95279 MW;  816B96D167B6F31C CRC64;
     MIVREGFFKS LKSIITLTVL LFSLSQAYAQ ELKLNDLEYF ETQGVNVLVY NNLFTGGFND
     EKNAGIELIH HGVRTVQGGA VRLSNTPEQW DLVPEISTRK VDRASKSIES TLRYKDYDFD
     SRVIVTAKGS GFEISVYLDK PIPKELEGSA GFNLEFLPSQ YWGKAYLADG RYNRFPRYVV
     GNSVARPNNE KPKQFKGYKT YDDRGTGKFI DPLPLERGRT FLLAPDEPSR LVKITSPDVD
     LMLFDGRVLA QNGWFVVRSI LPSGKTGKVL TWTVEPNAIK GWVREPNIGF SQVGYIPKQQ
     KVAVIELDKK DKPLATASLY SIGNDGSARE VFSGKIVSWG DYYKYHYVKF DFTSVTTPGI
     YYIKYGNFKT NDFLIENSVY DKITDATSDI WIPIHMDHMF VNEAYRVWHG EPFKEGYLQA
     PPNTDHFDLH AQGPTTDTKY KALEPIPGLN VGGFFDAGDF DIETGANISV VQNFVQTWEY
     FKPLRDQTFV DQKQRYVDLH RPDGTPDVLQ FIEHGTLNLV AQAEIVGHMT QTLSNAVLDN
     YHHLGDAASL TDGLPYNPNL GPYEKAKDGR SSGVKDDMWA FTSRNPRLDL YAATMFAAAS
     RALKGYNDDL SARALVQSKR LLKEATDLLA AKPEDNSPWG ASANVGTNLQ LYIATGEKQY
     RDKFQELLWP ALDRNVNTTL LTALNAVPHM DAAFKEKLRP YVVKYADYIK GLEKENPYGV
     PIVLGNWAGS GAILNFGTTI CFASQYFPDV VAPGEAFKAT NWLFGCHPYH NYSLVATVGA
     TRPKAVFYGN NRADFSFIPG NVAPGILFRK PDHFENYDDW PFLWGQNEGT IGGNTSYLIF
     GSAFKNLVK
//