ID A0A1M6CMA9_9CLOT Unreviewed; 584 AA.
AC A0A1M6CMA9;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Radical SAM superfamily enzyme YgiQ, UPF0313 family {ECO:0000313|EMBL:SHI61848.1};
GN ORFNames=SAMN02745176_00838 {ECO:0000313|EMBL:SHI61848.1};
OS Lutispora thermophila DSM 19022.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae; Lutispora.
OX NCBI_TaxID=1122184 {ECO:0000313|EMBL:SHI61848.1, ECO:0000313|Proteomes:UP000184442};
RN [1] {ECO:0000313|EMBL:SHI61848.1, ECO:0000313|Proteomes:UP000184442}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19022 {ECO:0000313|EMBL:SHI61848.1,
RC ECO:0000313|Proteomes:UP000184442};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; FQZS01000005; SHI61848.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M6CMA9; -.
DR STRING; 1122184.SAMN02745176_00838; -.
DR Proteomes; UP000184442; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000184442}.
FT DOMAIN 5..138
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 176..405
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 584 AA; 68557 MW; 675E6CD429EF28F7 CRC64;
MNNTVKISLI AINSKFIHSN LAVRYLKSYL HKKGIKADIL EFSINDSFDN IIRKIYQTNS
NILAFSCYIW NRELIMKLGA SLKKIMPHSK IVLGGPEVSY DIEYTMKENP WINFIIFGEG
EETFFQFANS FIKGDKNIDH LEGLAFNSNG IIQVNNSRSP ISDLDEIPFP YNDFADLEHK
IIYYETSRGC PFKCQYCMSS LETGVRYFSL ERVFRDIDLF ISNKVKQVKL VDRTFNCNKK
RSIEIMEYIL SKKGHTNFHF EISPKLIDSD FLYTVKKAPK GIFQFEIGIQ STNNDTLEAI
RRYERFDEIK EKISALIALE NCHIHLDLIA GLPFEDIKSF EKSFNNVFSL KPQMLQLGFL
KLLRGSGLRM DADKHGIQYN DHPPYEVLST KWLSYKDILD LKMIEELVEI FSNSGRFNKS
LSYLIDYYFS NPFKFFKELG TFYYKKNAYD KSLKISEHYE MLYFFVKNNI GITTMFNELL
KYDFFSSLEG NMPSFIKRYD HSQIKNTVSS FLKKDFNIDR FIPELKNMDI RQKLKYLKFE
IFDIDVLGDL KEGRYIVLLI KNPKSDEEKR AVSFDLDEFI ELSL
//
