ID   A0A1M6EX46_9ACTN        Unreviewed;      1187 AA.
AC   A0A1M6EX46;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=SAMN05421803_102445 {ECO:0000313|EMBL:SHI90015.1};
OS   Nocardiopsis flavescens.
OC   Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC   Nocardiopsaceae; Nocardiopsis.
OX   NCBI_TaxID=758803 {ECO:0000313|EMBL:SHI90015.1, ECO:0000313|Proteomes:UP000184452};
RN   [1] {ECO:0000313|EMBL:SHI90015.1, ECO:0000313|Proteomes:UP000184452}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.5723 {ECO:0000313|EMBL:SHI90015.1,
RC   ECO:0000313|Proteomes:UP000184452};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; FQZK01000002; SHI90015.1; -; Genomic_DNA.
DR   RefSeq; WP_073376384.1; NZ_FQZK01000002.1.
DR   AlphaFoldDB; A0A1M6EX46; -.
DR   STRING; 758803.SAMN05421803_102445; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000184452; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184452};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          6..73
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
SQ   SEQUENCE   1187 AA;  131164 MW;  8E5E89FEDE103EE3 CRC64;
     MADSFVHLHV HTEYSMLDGA AKLKPLFKEA GRLGMPAVAM TDHGNMFGAY EFYQQSKGSG
     VTPIIGIEAY VAPESRFHKK RVFWGRASGS DDASAEGGKD ISGGGRYLHM TMLARNATGL
     RNLFKMSSLA SVEGYYMKPR MDLDLMSENS EGIIVSTGCP SGGVQTRLRL GQEKEAIEYA
     AGLQDIFGKE NVFLELMDHG VPIEKEVRDG LLKVGRELKI PPLVTNDSHY VYESQADAHD
     ALLAVGVGKN LDDPTRFRFN GSGYYLKTAD EMRGLLTFDQ WEEGCKNTLL IAERVEPGAY
     DEVFAHRDLM PVFPVPEGET QSSWLAKEVE RLLPTRYPNG AGADVRERVE FELGIINTMG
     FPAYFLVVAD ICQYARKTGI ALGPGRGSAA GSMIAYVLGI TDLDPLEHGL LFERFLNPER
     VSMPDVDLDF DERRRGEMIE YVTRLYGSER VAQILTFGTI KAKAAVKDST RILGMPYSVG
     DQITKAFPAA VGGKEIPLDA VFNTEHERYA ETTELRNLVD NDPQVAKVME TARGIEGLTR
     GTGVHAAGVI LSKEPLLDVI PLHMRDADGA IITGFPYPQC EEMGLLKMDF LGLRNLTIMD
     DAVKSIKEAE GIDIDLSKLP LDDKKTYQLL QRGDTLGVFQ LDGGAMRNLL KRMQPKKFGD
     IAAVNALYRP GPMAANAHND YADRSNGRQD ITAIHPELKD ALDPILGETF HLIVYQEQIM
     AIAQQLAGYT LGGADLMRRA MGKKKKEALE AEEAKFFPGA MERGFSEEAI RTLWDVMLPF
     SGYAFNKSHA AGYALVSYWT AYLKANHPAA YMAALLTSVS DDKDKMAVYL AECRSQGIKV
     LPPDVNESGL RFTPVGKDIR FGMGAVRNVG ANVVNSIIKS RTEKGNYTSF TDFLSKIELA
     ACNKRVIESL VKSGAFDSLG QPRRELYRHH EIAVDGMISS KKQEAHGQFD LFGGADEGGD
     STPIGLNINW GDEEWDRKTK LAFEREMLGL YVSSHPLAGA ERVLARSRDT SIAQIVAGEM
     GRERNELRIA GLISKVEKRV NKAGNQWAIA TVEDLDASME VLFFPKTFPL YVDALIEDTA
     VTVKGRLNDR DGTWSMFASD MSILDISHIT EGEPPVLLTV DEQRLTRELV EELRQVLSSH
     KGDTPVRIRV DNPVRSKVFA VDRYSVRISP EFNGEMKSLL GAYAVDY
//