ID A0A1M6GD00_9FLAO Unreviewed; 484 AA.
AC A0A1M6GD00;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Probable membrane transporter protein {ECO:0000256|RuleBase:RU363041};
GN ORFNames=SAMN05444363_2539 {ECO:0000313|EMBL:SHJ07780.1};
OS Flavobacterium terrae.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=415425 {ECO:0000313|EMBL:SHJ07780.1, ECO:0000313|Proteomes:UP000184488};
RN [1] {ECO:0000313|Proteomes:UP000184488}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18829 {ECO:0000313|Proteomes:UP000184488};
RA Varghese N., Submissions S.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU363041};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU363041}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC (TC 2.A.102) family. {ECO:0000256|RuleBase:RU363041}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FQZI01000005; SHJ07780.1; -; Genomic_DNA.
DR RefSeq; WP_073311872.1; NZ_FQZI01000005.1.
DR AlphaFoldDB; A0A1M6GD00; -.
DR STRING; 415425.SAMN05444363_2539; -.
DR OrthoDB; 45564at2; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000184488; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR InterPro; IPR002781; TM_pro_TauE-like.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF01925; TauE; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU363041};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363041};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW Reference proteome {ECO:0000313|Proteomes:UP000184488};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363041};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363041}.
FT TRANSMEM 204..228
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 240..264
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 309..332
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 338..359
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 406..428
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 434..452
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 464..481
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT DOMAIN 125..150
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
SQ SEQUENCE 484 AA; 52612 MW; 9B3A10859B50FFEE CRC64;
MTNTLFPIFL KTETARFLIV GGGNVGLEKT ETLLRQNPNV SIKLIAIEIS LKVKEVLAQN
PHIVFHERPF EENDLKETDF VITATNDKVL NTQIKQKANQ LKILVNSADQ PDLCDFYLGS
IVKKGHLKIA ISTNGKSPVL ARRLREHLED TIPENINESI ENLNAFREKH KGDFQSKLAS
LNQVTSVLVD QTNSTKKEQN YKKLVFQIAG IFLAVFFGYG LSTIISVTDL NHLASNIPQA
FFAMIAVGFF AQIVDGAVGL GYGVTCATSM MLFGIKLPAI SGSIHTAEMF SSGISGFSHY
KFGNVNKKLL FWLAIPGAIG AVSGALLLIY LGDKYETITY GVLATYTMLI GIRLIVIAFR
EKIDKKQVKH TGVLGFSGGF FDAFGGGGWG PIVTSTLLAK GRKSKYVIGT VSLAEFFVTL
AASITFFASL GVSHWYIVVG LILGGSLAAP LAAKLAGKLP QKTAILLVAF LVIVFSVRML
LKIV
//
