ID A0A1M6GX10_9FIRM Unreviewed; 521 AA.
AC A0A1M6GX10;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN ORFNames=SAMN02746098_05235 {ECO:0000313|EMBL:SHJ14447.1};
OS Desulfosporosinus lacus DSM 15449.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=1121420 {ECO:0000313|EMBL:SHJ14447.1, ECO:0000313|Proteomes:UP000183954};
RN [1] {ECO:0000313|EMBL:SHJ14447.1, ECO:0000313|Proteomes:UP000183954}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15449 {ECO:0000313|EMBL:SHJ14447.1,
RC ECO:0000313|Proteomes:UP000183954};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
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DR EMBL; FQXJ01000040; SHJ14447.1; -; Genomic_DNA.
DR RefSeq; WP_073033450.1; NZ_FQXJ01000040.1.
DR AlphaFoldDB; A0A1M6GX10; -.
DR STRING; 1121420.SAMN02746098_05235; -.
DR OrthoDB; 9803687at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000183954; Unassembled WGS sequence.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR InterPro; IPR022026; MTHFR_C.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF12225; DUF5981; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862}.
FT DOMAIN 396..488
FT /note="Methylene-tetrahydrofolate reductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF12225"
SQ SEQUENCE 521 AA; 57964 MW; 332CBF5D1F4BB85E CRC64;
MENKFKESLL DKNTLSVTWE LVPGRGANEK AQLTAIASAE QAAKGGRVHA LTITDNPGGN
PAILADYLGM EILKLGIEPL VHFTCKDKNR NQMESQLYAL DRAKVRNLLV MTGDYTYSGF
KGRPKPVFDL DPSNTLQLIA DMNKGLEVPG MKGPTYHAPS DFFAGAAVSP FKSTEAELMV
QYYKLKKKIQ GGAQFVVTQL GYDARKCHEV IQFLKVNNLD IPVVGNIYVL PYGAANVMNQ
NKLPGCVVTD KLLAELNEEK SAADKGVGQR MLRAAKLYAI LKGIGCAGVH IGGHNLKYEQ
LEYILDKGEE LYPNWQDLVK EFDYPQTNGF YYFEKDEKTG LNTDRPTNRA NRPLDAPAGA
MNSLMRGMHS LMFTPNKMLF PVMRSMYHAA EGKKKAEKRL HALEHIAKVV MLDCKDCGDC
AILDIGYLCP MSQCPKNQRN GACGGSLDGW CEVFPKEKKC VWVRAYARLK KYGEESTLDV
PPVPPANWDF YQTSSWSNFY LGKDHSAPLL GIKPPESKIK K
//
