ID   A0A1M6ILA0_9PROT        Unreviewed;       790 AA.
AC   A0A1M6ILA0;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=Penicillin amidase {ECO:0000313|EMBL:SHJ35187.1};
GN   ORFNames=SAMN02745194_02374 {ECO:0000313|EMBL:SHJ35187.1};
OS   Roseomonas rosea.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Acetobacteraceae; Roseomonas.
OX   NCBI_TaxID=198092 {ECO:0000313|EMBL:SHJ35187.1, ECO:0000313|Proteomes:UP000184387};
RN   [1] {ECO:0000313|EMBL:SHJ35187.1, ECO:0000313|Proteomes:UP000184387}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14916 {ECO:0000313|EMBL:SHJ35187.1,
RC   ECO:0000313|Proteomes:UP000184387};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
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DR   EMBL; FQZF01000012; SHJ35187.1; -; Genomic_DNA.
DR   RefSeq; WP_073135037.1; NZ_FQZF01000012.1.
DR   AlphaFoldDB; A0A1M6ILA0; -.
DR   STRING; 198092.SAMN02745194_02374; -.
DR   OrthoDB; 9760084at2; -.
DR   Proteomes; UP000184387; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184387};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        269
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         209
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         342
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         345
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   790 AA;  85095 MW;  EB6E5AB3D1F7AF91 CRC64;
     MASPPRRVRP VIRHQGRHPG RHLGRWLRRT LLILVALPLL LLAAAAGAIW WTLPGEAEAL
     RLSGVSGPVE IGFDDRGIPR IRAASERDAA MALGVLHARD RMFQMEMTRR GGAGRLSEIA
     GASTLRLDRF MRTLGLRQRA EADLAALPAE ARDMLQAYAD GVNAWARARG RFVAPEFIAL
     GEPEPWRPAD SLLWGKVMGL WLSGNWRTET ERARLAGILS AERMEQLWPA DESLGRADLA
     ALPQPARLAS LLKAVPRWGE DAPLPNSASN AWAVAPPRSV TGGALLASDP HLGLQAPVLW
     YLARVELPGG RFLAGATAPG VPMIVIGRNE SLGWGFTTTH SDTQDVFVER LAGPDAYQTP
     DGPRPFTTRE ERIAVRGRPE EVLRVRETRH GPVISDLDEE PPGEQVLAVA MASLAPADTQ
     AAGLLALNRA RSVAEARAAA ALIASPAQNL MVADAAGGIA MFLTGRTPRR AAGDGTLPAP
     GWDGSHDWTG WVPFEEMPHV VAPPGGVIAN ANNRVQPAGS NVFLGRDWFG DWRFRRIGDL
     LRQRPRHAPG DFAAMQADAV SLLAREMLPL LLAPPRPEGM AGAARDLLPG WDGRMGPELP
     QPLVFNAWMR ELGRLAMAAG GVPEGSWTAG PEFLALLTRP EGAAWCGADG CEAMSMRALE
     AAVASLSPRF GPDPHRWRWG DAHRVRLEHP LLRFVPLLRD LIRIEAPTPG DGQTVNRGGM
     GRDFTHIHGP GLRIVMDLSS PDGVLAAIAT GQSGNPFSRH WGDRTAGWAA GQAEALPAIA
     PARARLRLDP
//