ID A0A1M6KKS8_9FIRM Unreviewed; 327 AA.
AC A0A1M6KKS8;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE SubName: Full=Endoglucanase {ECO:0000313|EMBL:SHJ59543.1};
GN ORFNames=SAMN02745227_00158 {ECO:0000313|EMBL:SHJ59543.1};
OS Anaerobranca californiensis DSM 14826.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Proteinivoraceae;
OC Anaerobranca.
OX NCBI_TaxID=1120989 {ECO:0000313|EMBL:SHJ59543.1, ECO:0000313|Proteomes:UP000243547};
RN [1] {ECO:0000313|Proteomes:UP000243547}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14826 {ECO:0000313|Proteomes:UP000243547};
RA Varghese N., Submissions S.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC Note=Binds 2 divalent metal cations per subunit.
CC {ECO:0000256|PIRSR:PIRSR001123-2};
CC -!- SIMILARITY: Belongs to the peptidase M42 family.
CC {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
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DR EMBL; FRAI01000005; SHJ59543.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M6KKS8; -.
DR STRING; 1120989.SAMN02745227_00158; -.
DR OrthoDB; 9772053at2; -.
DR Proteomes; UP000243547; Unassembled WGS sequence.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd05656; M42_Frv; 1.
DR Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR InterPro; IPR008007; Peptidase_M42.
DR InterPro; IPR023367; Peptidase_M42_dom2.
DR PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR PANTHER; PTHR32481:SF9; ENDOGLUCANASE; 1.
DR Pfam; PF05343; Peptidase_M42; 1.
DR PIRSF; PIRSF001123; PepA_GA; 1.
DR SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000243547}.
FT ACT_SITE 192
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT BINDING 60
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT BINDING 162
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT BINDING 162
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT BINDING 193
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT BINDING 215
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ SEQUENCE 327 AA; 36026 MW; D85CD5F4B3272124 CRC64;
MELLKRLSES FGPSGHETRV ANLIVEEIKG YCDEIFIDKL GNVIALKKGQ SDKKIMYASH
IDEIGLLVTY IEKEGFLRFT NIGGVSPYIL LGQRVKLDCG VIGVIGSEKI TDISKLTLDK
MFIDIGVNSK EEAEKLISIG EVAVIHREFE NKGDKVIGKA LDDRAGCWVL INALKQIKNP
EHNLYFVFTV QEEVGLRGAK TAAYRIDPDL AIAVDVTAVG DTPKAHRMAV ELGKGPAIKV
KDMSIMVHPM VKDMLIKKAR ELSIPYQLEV LEFGGTDSGA IHVSREGVWA GVVSIPCRYI
HTPSEMVSVD DLNNAVKLIV ASTEFEF
//
