ID   A0A1M6Q4I3_9FIRM        Unreviewed;       814 AA.
AC   A0A1M6Q4I3;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE            EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE   AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN   ORFNames=SAMN02745136_01860 {ECO:0000313|EMBL:SHK15037.1};
OS   Anaerocolumna jejuensis DSM 15929.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC   Anaerocolumna.
OX   NCBI_TaxID=1121322 {ECO:0000313|EMBL:SHK15037.1, ECO:0000313|Proteomes:UP000184386};
RN   [1] {ECO:0000313|EMBL:SHK15037.1, ECO:0000313|Proteomes:UP000184386}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15929 {ECO:0000313|EMBL:SHK15037.1,
RC   ECO:0000313|Proteomes:UP000184386};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC         in beta-D-mannosides.; EC=3.2.1.25;
CC         Evidence={ECO:0000256|ARBA:ARBA00000829};
CC   -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC       {ECO:0000256|ARBA:ARBA00004740}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC       mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FRAC01000009; SHK15037.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1M6Q4I3; -.
DR   STRING; 1121322.SAMN02745136_01860; -.
DR   Proteomes; UP000184386; Unassembled WGS sequence.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR041447; Mannosidase_ig.
DR   PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR   PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   Pfam; PF17786; Mannosidase_ig; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184386}.
FT   DOMAIN          69..127
FT                   /note="Glycosyl hydrolases family 2 sugar binding"
FT                   /evidence="ECO:0000259|Pfam:PF02837"
FT   DOMAIN          238..286
FT                   /note="Glycoside hydrolase family 2 immunoglobulin-like
FT                   beta-sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF00703"
FT   DOMAIN          642..729
FT                   /note="Mannosidase Ig/CBM-like"
FT                   /evidence="ECO:0000259|Pfam:PF17786"
SQ   SEQUENCE   814 AA;  94519 MW;  CEE7D3FF87FAECE9 CRC64;
     MVKRVMNDQF RIRIAGKDQW YPFSIPGSAM TTFVEQGVIP DPFDGMNEYE VKEFLRNDFE
     IEGILKVTPE EYQNQNMELV FHGVDTLADI YINDKKLGHV ENMHRSYVFP CKEWIKEGEN
     KLSVKFFSAL EFIESRKPAK GREITMANTG TLLGEQYLRK AHCMFGWDWG PQLPDCGIYG
     NIEFCAYGEA KLGETLIRQA WTKEQVTLLL STEISGGTDT KAEICYRVLD PAGACLYHGT
     KTEISIEKPE LWWPNGYGKQ PLYSVEAMLN IPGEEPQKKT YTIGLRRLTV SQEEDQWGKE
     FAFMVNGVKI FARGADYIPD DCFYPKITHE VLKRDVEAAV YANFNSLRVW GGGYYPSDDF
     YELCDKYGII LWQDFMYACN VYDLDDAFIE NIVEEAKDNL IRFRNHPCLG LICGNNEMEV
     AWLEWKDMKD HAPSLKKDYL LQFEYYLANA VAEYAPDVFY WPSSPSSGGS FDDPGDENRG
     DSHYWDVWHG QKPYTEYRKH FFRFCSEFGF QSLPSIKTIE AFTKEEDRNL FSRVMESHQK
     NPAANGKILY YLSETFRYPK NLENLVFLSQ VIQGYAIKSG VEHWRRNRGR CMGSIYWQFN
     DNWPVASWSS MDYYGRYKAL HYMAREFYAP VAGSIEREGT VCRFWISNET TEPCQIKGRI
     TLKTLDFEVL DQIQVSETID SLSAACIFTK DYQSLIEGRD DSVFVMMEYQ YGEEQQGREE
     WEVFVPVKYL KLEDPELQLT VNAENQLELT AKSFAPYCML EDKKADRILN KNVFAVTAAS
     PVTLELCKTE NALEPDAMDA QQWKIYDVYH TYEQ
//