ID A0A1M6QLG7_9BACT Unreviewed; 547 AA.
AC A0A1M6QLG7;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=CTP synthase (glutamine hydrolyzing) {ECO:0000256|ARBA:ARBA00012291};
DE EC=6.3.4.2 {ECO:0000256|ARBA:ARBA00012291};
GN ORFNames=SAMN05720469_102152 {ECO:0000313|EMBL:SHK21129.1};
OS Fibrobacter intestinalis.
OC Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales; Fibrobacteraceae;
OC Fibrobacter.
OX NCBI_TaxID=28122 {ECO:0000313|EMBL:SHK21129.1, ECO:0000313|Proteomes:UP000184275};
RN [1] {ECO:0000313|Proteomes:UP000184275}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UWOS {ECO:0000313|Proteomes:UP000184275};
RA Varghese N., Submissions S.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000314};
CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC CTP from UDP: step 2/2. {ECO:0000256|ARBA:ARBA00005171}.
CC -!- SIMILARITY: Belongs to the CTP synthase family.
CC {ECO:0000256|ARBA:ARBA00007533}.
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DR EMBL; FRAW01000002; SHK21129.1; -; Genomic_DNA.
DR RefSeq; WP_073302201.1; NZ_FRAW01000002.1.
DR AlphaFoldDB; A0A1M6QLG7; -.
DR UniPathway; UPA00159; UER00277.
DR Proteomes; UP000184275; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003883; F:CTP synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd03113; CTPS_N; 1.
DR CDD; cd01746; GATase1_CTP_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR004468; CTP_synthase.
DR InterPro; IPR017456; CTP_synthase_N.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR033828; GATase1_CTP_Synthase.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00337; PyrG; 1.
DR PANTHER; PTHR11550; CTP SYNTHASE; 1.
DR PANTHER; PTHR11550:SF0; CTP SYNTHASE-RELATED; 1.
DR Pfam; PF06418; CTP_synth_N; 1.
DR Pfam; PF00117; GATase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000256|ARBA:ARBA00022598}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..37
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 12..275
FT /note="CTP synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF06418"
FT DOMAIN 314..541
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT ACT_SITE 394
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 522
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 524
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 547 AA; 61284 MW; 96E172684C803415 CRC64;
MKKEHEYNGK TKFIVVTGGV ISGLGKGVAA ASIGALLSNR LKVIPVKCDG YLNTDPGTMN
PTEHGEVYVL DDGGEVDMDF GHYERFLNVV ARSDWSLTMG KVFKLILEKE RRGDFLGHTV
QFIPHVTNVI KEHFYEVANS ENADVLLIEI GGTVGDLENQ FFIEAARQLS HDVGSENCMF
VHLTYVPIPS GVKEQKSKPT QMSVRELNER GIYPDIIIAR CEEFLKPHIK EKIGLFCNLP
ASHVISGVDV KHVYECPLVY DKEGVPEILL KKLRIYAPPK LDRWSRLVDT MNRNDVSPRK
VLTVAIGGKY TRLEDSYASI VESLNHVSAH LDVHVEIRWI DTEKLEKHPE EVEKDMQGID
AVIVPGGFGT RGIEGKIVLI QYVREHKIPF LGICYGMQLA VVEFARHVCG MAEAGTMETE
ADSHVKDPVI AYLPGQDKIR EMGASMRLGG HDVLISKGSL AERVYGATEI RERFRHRYEV
NPEYVERLEK GGIIFSGKAK NENIMQILEL ADHPFFMACQ FHPELKSSLL KPAPLFLGLL
KAVDERG
//
