ID   A0A1M6SX43_PSETH        Unreviewed;       538 AA.
AC   A0A1M6SX43;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   RecName: Full=CTP synthase (glutamine hydrolyzing) {ECO:0000256|ARBA:ARBA00012291};
DE            EC=6.3.4.2 {ECO:0000256|ARBA:ARBA00012291};
GN   ORFNames=SAMN05443637_10710 {ECO:0000313|EMBL:SHK49256.1};
OS   Pseudonocardia thermophila.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Pseudonocardia.
OX   NCBI_TaxID=1848 {ECO:0000313|EMBL:SHK49256.1, ECO:0000313|Proteomes:UP000184363};
RN   [1] {ECO:0000313|EMBL:SHK49256.1, ECO:0000313|Proteomes:UP000184363}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 43832 {ECO:0000313|EMBL:SHK49256.1,
RC   ECO:0000313|Proteomes:UP000184363};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC         glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC         ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00000314};
CC   -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC       CTP from UDP: step 2/2. {ECO:0000256|ARBA:ARBA00005171}.
CC   -!- SIMILARITY: Belongs to the CTP synthase family.
CC       {ECO:0000256|ARBA:ARBA00007533}.
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DR   EMBL; FRAP01000007; SHK49256.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1M6SX43; -.
DR   STRING; 1848.SAMN05443637_10710; -.
DR   UniPathway; UPA00159; UER00277.
DR   Proteomes; UP000184363; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003883; F:CTP synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd03113; CTPS_N; 1.
DR   CDD; cd01746; GATase1_CTP_Synthase; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR004468; CTP_synthase.
DR   InterPro; IPR017456; CTP_synthase_N.
DR   InterPro; IPR017926; GATASE.
DR   InterPro; IPR033828; GATase1_CTP_Synthase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   NCBIfam; TIGR00337; PyrG; 1.
DR   PANTHER; PTHR11550; CTP SYNTHASE; 1.
DR   PANTHER; PTHR11550:SF0; CTP SYNTHASE-RELATED; 1.
DR   Pfam; PF06418; CTP_synth_N; 1.
DR   Pfam; PF00117; GATase; 1.
DR   SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51273; GATASE_TYPE_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW   ECO:0000256|PROSITE-ProRule:PRU00605};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184363}.
FT   DOMAIN          1..235
FT                   /note="CTP synthase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF06418"
FT   DOMAIN          270..501
FT                   /note="Glutamine amidotransferase"
FT                   /evidence="ECO:0000259|Pfam:PF00117"
FT   ACT_SITE        350
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT   ACT_SITE        482
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT   ACT_SITE        484
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ   SEQUENCE   538 AA;  58156 MW;  E2EA36643F8F5088 CRC64;
     MQKLDPYLNV DPGTMNPFQH GEVFVTEDGA ETDLDIGHYE RFLDRDLTGR ANVTTGQVYS
     AVIAKERRGE YLGDTVQVIP HITNEIKERI LAMAEPGPDG VAPDVVITEV GGTVGDIESL
     PFLEAARQVR HDVGRDNCFF LHVSLVPYLA PSGELKTKPT QHSVAALRNI GIQPDGLVLR
     ADRDIPEPMK RKISLMCDVE QDGVVAAPDA PSIYDIPRVL HREGLDAYVV RRLGLPFRDV
     DWTVWGDLLD RVHKPEETTR IALVGKYVDL PDAYLSVTEA LRAGGFAHRA RVEIVWVPSD
     LCETPAGAAA ALADVDGVLI PGGFGVRGIE GKVGAVRHAR LNKIPALGLC LGLQCMVIEV
     ARSLAGLEGA NSAEFDENTP HPVISTMADQ RDVVAGERDM GGTMRLGAYP ATLLAGSVVA
     AAYGTREVSE RHRHRYEVNN AYRGKLEEAG LVFGGTSPDG KLVEFVELPQ DVHPFFVGTQ
     AHPELKSRPT RPHPLFAAFV KAALDYQAAG RLPVELPARD RVPVNTAASA AVNTTGAS
//