ID A0A1M6TJ41_9AQUI Unreviewed; 659 AA.
AC A0A1M6TJ41;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=SAMN05444391_1485 {ECO:0000313|EMBL:SHK56967.1};
OS Thermocrinis minervae.
OC Bacteria; Aquificota; Aquificae; Aquificales; Aquificaceae; Thermocrinis.
OX NCBI_TaxID=381751 {ECO:0000313|EMBL:SHK56967.1, ECO:0000313|Proteomes:UP000189810};
RN [1] {ECO:0000313|EMBL:SHK56967.1, ECO:0000313|Proteomes:UP000189810}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19557 {ECO:0000313|EMBL:SHK56967.1,
RC ECO:0000313|Proteomes:UP000189810};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
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DR EMBL; LT670846; SHK56967.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1M6TJ41; -.
DR STRING; 381751.SAMN05444391_1485; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000189810; Chromosome i.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01585; AcnA_Bact; 1.
DR CDD; cd01579; AcnA_Bact_Swivel; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000189810}.
FT DOMAIN 9..408
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 513..586
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 659 AA; 71972 MW; A245933FBE7B73A7 CRC64;
MAKGTVAYKI LQSHLVSGKL IPGEEIAIKI DQTLTQDATG TMAYLQFEAM GVDRVKTELS
VSYIDHNMLQ TDFKNPDDHK YLMTVAKRYG IYLSKPGNGI CHQVHLERFA KPGKTLLGSD
SHTPTAGGMG MLAIGAGGLD VAAAMAGEPF YLKMPRIVGV KLTGKLPDWV TAKDIILELL
RRLSVKGGVG KIFEYFGEGI KELSVPERAT ITNMGAELGA TTSIFPSDEI TRAYLRAQGR
EHDWIELLPD PDAEYDEIIE INLSELEPLI ACPHSPDNVV PVREVEGIKV DQVVIGSCTN
SSFVDLTRAG KLLEGRKVHP DVIFAVAPGS KQALELITQN GILLNFLKAG ARILESACGP
CIGMGFAPPS GGVSLRSFNR NFEGRSGTPD AKVYLASPEV CVAAAIAGHI IDPRKLAEKE
GIKWIRVEMP ERFPYGDEAI IEPLSEEEAK KVEIYRGPNI KPLPEFDSLP ESIDGVASLI
VGDNITTDHI MPAGAKILPL RSNIYAISEY VYHYVDPEFV SRAKQVRDSG RANVIIGGEN
YGQGSSREHA ALAPRFLGVR VVIAKSFARI HHANLVNFGV LPLEFVDKKD YEKFTLGDEV
LIPDLIQRLQ EGKEIIVVNK TTGEEIVCKY NLTPKQISVL MAGGLLNWIK NKQKVGVQT
//
