ID A0A1M7EFF5_9RHOB Unreviewed; 414 AA.
AC A0A1M7EFF5;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044150};
DE EC=1.5.3.24 {ECO:0000256|ARBA:ARBA00044044};
DE AltName: Full=Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta {ECO:0000256|ARBA:ARBA00044216};
DE AltName: Full=Tetrameric sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044295};
GN ORFNames=SAMN05444398_10751 {ECO:0000313|EMBL:SHL90515.1};
OS Roseovarius pacificus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Roseovarius.
OX NCBI_TaxID=337701 {ECO:0000313|EMBL:SHL90515.1, ECO:0000313|Proteomes:UP000183974};
RN [1] {ECO:0000313|EMBL:SHL90515.1, ECO:0000313|Proteomes:UP000183974}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 29589 {ECO:0000313|EMBL:SHL90515.1,
RC ECO:0000313|Proteomes:UP000183974};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2;
CC Xref=Rhea:RHEA:70455, ChEBI:CHEBI:15379, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:57305, ChEBI:CHEBI:57433,
CC ChEBI:CHEBI:57453; EC=1.5.3.24;
CC Evidence={ECO:0000256|ARBA:ARBA00043745};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; Evidence={ECO:0000256|ARBA:ARBA00043809};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the SoxB family.
CC {ECO:0000256|ARBA:ARBA00043973}.
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DR EMBL; FRBR01000007; SHL90515.1; -; Genomic_DNA.
DR RefSeq; WP_073035176.1; NZ_FRBR01000007.1.
DR AlphaFoldDB; A0A1M7EFF5; -.
DR STRING; 337701.SAMN05444398_10751; -.
DR OrthoDB; 9815989at2; -.
DR Proteomes; UP000183974; Unassembled WGS sequence.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR006278; SoxB.
DR NCBIfam; TIGR01373; soxB; 1.
DR PANTHER; PTHR13847:SF287; FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR13847; SARCOSINE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022643};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Reference proteome {ECO:0000313|Proteomes:UP000183974}.
FT DOMAIN 35..79
FT /note="Rhodanese"
FT /evidence="ECO:0000259|PROSITE:PS50206"
SQ SEQUENCE 414 AA; 44953 MW; 0741D2EF4940072A CRC64;
MKRYSAFAVA REALRYHMGW DRAWASPEPK KKYDVIIVGA GGHGLATAYY LGKNFGITNV
AVLEKGWLGG GNTGRNTTII RSNYLQDSSA AIYEKARGLY ETLSQDLNYN IMYSPRGVMM
LAQTQHEVRG YERTAHANAL QGVATEFISP NRVKELVPII NIDGPRYPVL GALWQPRGGT
ARHDAVAWGY ARACSAMGMD IIQKCEVTGI SREGSKVTGV TTNRGDIACD KLGIVVAGHS
GHLADMAGFR LPIESVALQA LVSEPIKPCM DVVVMANTVH GYMSQSDKGE MVIGGGADGY
NNYTQRGSFH HVEETVRALV ETFPMISRLK MLRQWGGIVD MTGDRSPILS KTPVEGVFIN
CGWGTGGFKS IPGSGWGFAE LMAKGHSPLT AEFNLDRFKE GRFIDESVAA GVAH
//
