ID A0A1N6RAL8_9SPIO Unreviewed; 468 AA.
AC A0A1N6RAL8;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=23S rRNA m(5)U-1939 methyltransferase {ECO:0000313|EMBL:SIQ25878.1};
GN ORFNames=SAMN05920897_10628 {ECO:0000313|EMBL:SIQ25878.1};
OS Alkalispirochaeta americana.
OC Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Spirochaetaceae;
OC Alkalispirochaeta.
OX NCBI_TaxID=159291 {ECO:0000313|EMBL:SIQ25878.1, ECO:0000313|Proteomes:UP000186400};
RN [1] {ECO:0000313|EMBL:SIQ25878.1, ECO:0000313|Proteomes:UP000186400}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ASpG1 {ECO:0000313|EMBL:SIQ25878.1,
RC ECO:0000313|Proteomes:UP000186400};
RA Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
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DR EMBL; FTMS01000006; SIQ25878.1; -; Genomic_DNA.
DR RefSeq; WP_076488319.1; NZ_FTMS01000006.1.
DR AlphaFoldDB; A0A1N6RAL8; -.
DR STRING; 159291.SAMN05920897_10628; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000186400; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000186400};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 1..62
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 426
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 426
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 301
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 330
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 351
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 399
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 468 AA; 52518 MW; 153E2463904215F3 CRC64;
MKRELTGIRI SRVDSRGRGR GIHTLSDGQE RSVAVPGALP GDLIDVGVRG RKAGTIQGCL
LALHETSHPR REPRCPHFGS CGGCTLQDIS YADQLLLKQG TVEAAFREAE LTELLPEMTP
ILASPREFLY RNKLEFSFGA ERWLDEEEIL RAEAIPDRRG LGFHAPGRFD RVLDLRECHL
QPDPSEAIRS FLGDLARERG YSFYHAREHR GFLRLLIIRT SLTGDLMVTI MFGEDDQQQR
EELLQALGEA FPEITSLNYV VNTSKNDSIY PHQVVLWRGE PFITEQCGDL RLRIHPKAFY
QTNPEQALRL YQAAVALAHL RPEDLLFDLY SGIGSIALYA AGRVRRVVGI ESVEDAVEAA
RENARGNGIG NALFESGEVE KILPQVISRE GAPRVVMVDP PRAGMHPAAR KALATLAPET
IVYISCNPRT QAADLREFIP NYEIAHLQPV DMFPQTRHVE NIVLLRRR
//
