ID A0A1N6W2D1_9ACTN Unreviewed; 1085 AA.
AC A0A1N6W2D1;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=SAMN05421833_10454 {ECO:0000313|EMBL:SIQ84198.1};
OS Microbispora rosea.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Streptosporangiaceae; Microbispora.
OX NCBI_TaxID=58117 {ECO:0000313|EMBL:SIQ84198.1, ECO:0000313|Proteomes:UP000186096};
RN [1] {ECO:0000313|EMBL:SIQ84198.1, ECO:0000313|Proteomes:UP000186096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 12950 {ECO:0000313|EMBL:SIQ84198.1,
RC ECO:0000313|Proteomes:UP000186096};
RA Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FTNI01000004; SIQ84198.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1N6W2D1; -.
DR STRING; 58117.SAMN05421833_10454; -.
DR OrthoDB; 5240387at2; -.
DR Proteomes; UP000186096; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 14..338
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 339..670
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 949..976
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1085 AA; 118430 MW; 896F40FF61CE7312 CRC64;
MLTPGELAGR LGILPPTPEQ SAVIEAPPEP MVVVAGAGSG KSETMAGRVV WLVANGLARP
EQILGLTFTR KAAAELAERV RQRLAGLAAA GLVEPELLDS EPTVSTYHAY AARLVTDHAL
REGLEPTMRL VTPAVAWQMA ARVVGQYDGP MDRVDLSPPS VTAAVLELAG ELAEHLRDPA
DVREVGDWLR ERLASVTGRI VKDQRKPVET QAVREQLLPM VEAYERLKRG REVIDYGDQM
ALAARIASRH PEVGASERSR FSVVLLDEYQ DTSHAQLVLL RSLYGGGHPV TAVGDPCQSI
YGWRGASAGN LSRFPTDFPA ASGDPAQVRR LSVSFRNGEH VLGVAARIQA PLRHEAREVP
VLVPGGNRVG RGRVLCAFHE TAEDEAEWIA EGLAGMLHGE GAPDGLPWGE GERAKRGPAL
QPQDVAILAR KRSQFPALRR AMEARGIPVE VVGLGGLLTV PEVADIVATL RALYDPTAGD
ALVRLLAGPR RRIGPADLKV LGDLARELNK EIRTARGGPR PEDPLDQVVA DLAEERGSLI
DALDELPDRP DWLERFSPLA RMRLPAVARE LRQLRAHAGQ PLPDLISEIE RRLGLDVEVA
ARGGSPLAAR ADLDAFLDAA SRFAGDSEDP TLGAFLAYLK AAESEEFGLE AGRVGDSNTV
KLMTVHASKG LEWPVVVVPG LSQLTSQKGG LIKGSMFPAT PVTNSRWTEN PRKLPYPLRG
DSADLPELAG LDREALGAFD ERCRDRDLME ERRLAYVAVT RAHYLLIASG YRWGTSSRPL
EPSDFLREIR EAGARVVRWA EDPESSDNPL LAEPPVAEWP SVTVRDGILD GARLVEEAMM
GDAPPASADG RALLRDRDRE RLRAWARDTD LLLRERETNR QRAGAVVELP SRLSVSSLVT
LAQDPAAFAR QVRRPVPRKP APLARRGTTF HRWLESRWGQ QRLIDDLELP GAADELSETE
IQLAELQERF EESEWATREP VDLEVPFETM IGDRLVRGRM DAVFREGDGY VVIDWKTGER
PQGRAAETAS VQLAAYRVAW AALAGAPLEK VGAAFHYVRS NDTVRPVDLL DADGLTALIQ
RVPQD
//
