ID A0A1N7SDH7_9BURK Unreviewed; 865 AA.
AC A0A1N7SDH7;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN Name=acnA {ECO:0000313|EMBL:SIT45445.1};
GN ORFNames=BN2475_550048 {ECO:0000313|EMBL:SIT45445.1};
OS Paraburkholderia ribeironis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Paraburkholderia.
OX NCBI_TaxID=1247936 {ECO:0000313|EMBL:SIT45445.1, ECO:0000313|Proteomes:UP000187012};
RN [1] {ECO:0000313|EMBL:SIT45445.1, ECO:0000313|Proteomes:UP000187012}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=STM7296 {ECO:0000313|EMBL:SIT45445.1,
RC ECO:0000313|Proteomes:UP000187012};
RA Song W.-J., Kurnit D.M.;
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; CYGX02000055; SIT45445.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1N7SDH7; -.
DR STRING; 1247936.BN2475_550048; -.
DR OrthoDB; 9764318at2; -.
DR Proteomes; UP000187012; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lyase {ECO:0000313|EMBL:SIT45445.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000187012}.
FT DOMAIN 66..533
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 657..788
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 865 AA; 94344 MW; F91F9A1F3041276F CRC64;
MNTANRKRLP GTQLDFFDTR AAVDAIEPGA YDKLPYTSRV LAENLVRRCD PATLTASLRQ
IIERKRELDF PWFPARVVCH DILGQTALVD LAGLRDAIAA QGGDPALVNP VVPTQLVVDH
SLAVECGGFD PDAFAKNRAI EDRRNEDRFD FINWTKRAFR NVDVIPPGNG ILHQINLERM
SPVVQVKDGV AFPDTLVGTD SHTPMVDALG VIAIGVGGLE AESVMLGRAS YMRLPDIVGV
ELTGKPGEGI TATDVVLSLT EFLRKEKVVG AYLEFYGEGT ARLTLGDRAT IANMAPEFGA
TAAMFYIDEQ TIKYLKLTGR DDELVKLVET YAKHTGLWAD TLKHAEYERV LKFDLSSVVR
TLAGPSNPHR RLPVAELAAR GISGKVENEP GRMPDGAVII AAITSCTNTN NPRNMIAAGL
LARNANRRGL TRKPWAKTSL APGSKAVTLY LEEAGLLPEL EQLGFGVVAY ACTSCNGMSG
ALDPAIQKEV VERDLYATAV LSGNRNFDGR IHPYAKQAFL ASPPLVVAYA IAGTIRFDIE
KDVLGVDADG HAVTLKDIWP SDEEIDAIVA SSVKPAQFRK VYEPMFAVSA DTGVQADPLY
DWREMSTYIR RPPYWEGALA GERTLKGMRP LAVLGDNITT DHLSPSNAIL LDSAAGEYLA
KMGLPEEDFN SYATHRGDHL TAQRATFANP TLKNEMVFED GEVKAGSLAR IEPEGKITRM
WEAIETYMER KQPLIVIAGA DYGQGSSRDW AAKGVRLAGT EAIVAEGFER IHRTNLVGMG
VLPLEFKPGV NRITLGIDGT ETFDVIGERK PRADLTLVIQ RRNGERVEVP VTCRLDTAEE
VSIYEAGGVL QRFAQDFLES TKAAA
//
