ID A0A1P8B178_ARATH Unreviewed; 244 AA.
AC A0A1P8B178;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 13-SEP-2023, entry version 33.
DE SubName: Full=DNA-directed RNA polymerase family protein {ECO:0000313|EMBL:ANM62660.1};
GN Name=NRPB3 {ECO:0000313|EMBL:ANM62660.1, ECO:0000313|TAIR:AT2G15430};
GN Synonyms=NRPD3 {ECO:0000313|EMBL:ANM62660.1}, NRPE3A
GN {ECO:0000313|EMBL:ANM62660.1}, RBP36A {ECO:0000313|EMBL:ANM62660.1},
GN RPB35.5A {ECO:0000313|EMBL:ANM62660.1};
GN OrderedLocusNames=At2g15430 {ECO:0000313|Araport:AT2G15430,
GN ECO:0000313|EMBL:ANM62660.1};
GN ORFNames=F26H6.5 {ECO:0000313|EMBL:ANM62660.1}, F26H6_5
GN {ECO:0000313|EMBL:ANM62660.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:ANM62660.1, ECO:0000313|Proteomes:UP000006548};
RN [1] {ECO:0000313|EMBL:ANM62660.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S., Shea T.P., Benito M.I., Town C.D.,
RA Fujii C.Y., Mason T., Bowman C.L., Barnstead M., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2] {ECO:0007829|PubMed:22223895}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.M111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [3] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- SIMILARITY: Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase
CC subunit family. {ECO:0000256|ARBA:ARBA00025804}.
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DR EMBL; CP002685; ANM62660.1; -; Genomic_DNA.
DR RefSeq; NP_001324802.1; NM_001335451.1.
DR AlphaFoldDB; A0A1P8B178; -.
DR SMR; A0A1P8B178; -.
DR ProteomicsDB; 215938; -.
DR EnsemblPlants; AT2G15430.2; AT2G15430.2; AT2G15430.
DR GeneID; 816035; -.
DR Gramene; AT2G15430.2; AT2G15430.2; AT2G15430.
DR Araport; AT2G15430; -.
DR TAIR; AT2G15430; NRPB3.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; A0A1P8B178; baseline and differential.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd07031; RNAP_II_RPB3; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00320; RNApol_arch_Rpo3; 1.
DR InterPro; IPR001514; DNA-dir_RNA_pol_30-40kDasu_CS.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR022842; RNAP_Rpo3/Rpb3/RPAC1.
DR InterPro; IPR036643; RNApol_insert_sf.
DR PANTHER; PTHR11800; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR11800:SF2; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
DR PROSITE; PS00446; RNA_POL_D_30KD; 1.
PE 1: Evidence at protein level;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:ANM62660.1};
KW Proteomics identification {ECO:0007829|PeptideAtlas:A0A1P8B178,
KW ECO:0007829|ProteomicsDB:A0A1P8B178};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW Transcription {ECO:0000256|ARBA:ARBA00023163}.
FT DOMAIN 21..236
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
SQ SEQUENCE 244 AA; 27354 MW; 3A5CC607E3A13432 CRC64;
MDGATYQRFP KIKIRELKDD YAKFELRETD VSMANALRRV MISEVPTVAI DLVEIEVNSS
VLNDEFIAHR LGLIPLTSER AMSMRFSRDC DACDGDGQCE FCSVEFRLSS KCVTDQTLDV
TSRDLYSADP TVTPVDFTID SSVSDSSEHK GIIIVKLRRG QELKLRAIAR KGIGKDHAKW
SPAATVTFMY EPDIIINEDM MDTLSDEEKI DLIESSPTKV FGMDPVTRQI ALLSSLGELR
QSSE
//
