ID A0A1P8B1N1_ARATH Unreviewed; 433 AA.
AC A0A1P8B1N1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=phosphopyruvate hydratase {ECO:0000256|ARBA:ARBA00012058};
DE EC=4.2.1.11 {ECO:0000256|ARBA:ARBA00012058};
GN Name=LOS2 {ECO:0000313|EMBL:ANM62807.1, ECO:0000313|TAIR:AT2G36530};
GN Synonyms=cytosolic enolase {ECO:0000313|EMBL:ANM62807.1}, ENO2
GN {ECO:0000313|EMBL:ANM62807.1}, ENOC {ECO:0000313|EMBL:ANM62807.1},
GN ENOLASE 2 {ECO:0000313|EMBL:ANM62807.1};
GN OrderedLocusNames=At2g36530 {ECO:0000313|Araport:AT2G36530,
GN ECO:0000313|EMBL:ANM62807.1};
GN ORFNames=F1O11.16 {ECO:0000313|EMBL:ANM62807.1}, F1O11_16
GN {ECO:0000313|EMBL:ANM62807.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:ANM62807.1, ECO:0000313|Proteomes:UP000006548};
RN [1] {ECO:0000313|EMBL:ANM62807.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S., Shea T.P., Benito M.I., Town C.D.,
RA Fujii C.Y., Mason T., Bowman C.L., Barnstead M., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2] {ECO:0007829|PubMed:19245862}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [3] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 4/5. {ECO:0000256|ARBA:ARBA00005031}.
CC -!- SIMILARITY: Belongs to the enolase family.
CC {ECO:0000256|ARBA:ARBA00009604}.
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DR EMBL; CP002685; ANM62807.1; -; Genomic_DNA.
DR RefSeq; NP_001324936.1; NM_001336601.1.
DR AlphaFoldDB; A0A1P8B1N1; -.
DR SMR; A0A1P8B1N1; -.
DR EnsemblPlants; AT2G36530.2; AT2G36530.2; AT2G36530.
DR GeneID; 818226; -.
DR Gramene; AT2G36530.2; AT2G36530.2; AT2G36530.
DR Araport; AT2G36530; -.
DR TAIR; AT2G36530; LOS2.
DR UniPathway; UPA00109; UER00187.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; A0A1P8B1N1; baseline and differential.
DR GO; GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0004634; F:phosphopyruvate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03313; enolase; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR HAMAP; MF_00318; Enolase; 1.
DR InterPro; IPR000941; Enolase.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR020810; Enolase_C.
DR InterPro; IPR020809; Enolase_CS.
DR InterPro; IPR020811; Enolase_N.
DR NCBIfam; TIGR01060; eno; 1.
DR PANTHER; PTHR11902:SF59; BIFUNCTIONAL ENOLASE 2_TRANSCRIPTIONAL ACTIVATOR; 1.
DR PANTHER; PTHR11902; ENOLASE; 1.
DR Pfam; PF00113; Enolase_C; 1.
DR Pfam; PF03952; Enolase_N; 1.
DR PIRSF; PIRSF001400; Enolase; 1.
DR PRINTS; PR00148; ENOLASE.
DR SFLD; SFLDF00002; enolase; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SMART; SM01192; Enolase_C; 1.
DR SMART; SM01193; Enolase_N; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
DR PROSITE; PS00164; ENOLASE; 1.
PE 1: Evidence at protein level;
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Proteomics identification {ECO:0007829|PeptideAtlas:A0A1P8B1N1,
KW ECO:0007829|ProteomicsDB:A0A1P8B1N1};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548}.
FT DOMAIN 6..128
FT /note="Enolase N-terminal"
FT /evidence="ECO:0000259|SMART:SM01193"
FT DOMAIN 136..429
FT /note="Enolase C-terminal TIM barrel"
FT /evidence="ECO:0000259|SMART:SM01192"
FT ACT_SITE 204
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT ACT_SITE 341
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT BINDING 152
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 161
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 289
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 316
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 368..371
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 392
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
SQ SEQUENCE 433 AA; 46517 MW; 607D8C1808458F19 CRC64;
MIGDLISAIW IVDIHTSNGI KVTAAVPSGA STGIYEALEL RDGGSDYLGK GVSKAVGNVN
NIIGPALIGK DPTQQTAIDN FMVHELDGTQ NEWGWCKQKL GANAILAVSL AVCKAGAVVS
GIPLYKHIAN LAGNPKIVLP VPAFNVINGG SHAGNKLAMQ EFMILPVGAA SFKEAMKMGV
EVYHHLKSVI KKKYGQDATN VGDEGGFAPN IQENKEGLEL LKTAIEKAGY TGKVVIGMDV
AASEFYSEDK TYDLNFKEEN NNGSQKISGD ALKDLYKSFV AEYPIVSIED PFDQDDWEHY
AKMTTECGTE VQIVGDDLLV TNPKRVAKAI AEKSCNALLL KVNQIGSVTE SIEAVKMSKK
AGWGVMTSHR SGETEDTFIA DLAVGLSTGQ IKTGAPCRSE RLAKYNQLLR IEEELGSEAI
YAGVNFRKPV EPY
//
