ID A0A1P8BEG1_ARATH Unreviewed; 1086 AA.
AC A0A1P8BEG1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Insulinase (Peptidase family M16) family protein {ECO:0000313|EMBL:ANM69980.1};
GN Name=SPP {ECO:0000313|EMBL:ANM69980.1, ECO:0000313|TAIR:AT5G42390};
GN Synonyms=MDH9.8 {ECO:0000313|EMBL:ANM69980.1}, MDH9_8
GN {ECO:0000313|EMBL:ANM69980.1}, stromal processing peptidase
GN {ECO:0000313|EMBL:ANM69980.1};
GN OrderedLocusNames=At5g42390 {ECO:0000313|Araport:AT5G42390,
GN ECO:0000313|EMBL:ANM69980.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:ANM69980.1, ECO:0000313|Proteomes:UP000006548};
RN [1] {ECO:0000313|EMBL:ANM69980.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=11130714; DOI=10.1038/35048507;
RG Kazusa DNA Research Institute;
RG Cold Spring Harbor and Washington University in St Louis Sequencing Consortium;
RG European Union Arabidopsis Genome Sequencing Consortium;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., See L.H., Vil D., Baker J.,
RA Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Dusterhoft A., Stiekema W., Pohl T., Entian K.D.,
RA Terryn N., Hartley N., Bent E., Johnson S., Langham S.A., McCullagh B.,
RA Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H.,
RA Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Lankhorst R.K., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S.,
RA Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J.,
RA Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K., Rudd S., Schoof H.,
RA Schueller C., Zaccaria P., Mewes H.W., Bevan M., Fransz P.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- SIMILARITY: Belongs to the peptidase M16 family.
CC {ECO:0000256|ARBA:ARBA00007261}.
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DR EMBL; CP002688; ANM69980.1; -; Genomic_DNA.
DR RefSeq; NP_001331624.1; NM_001344443.1.
DR AlphaFoldDB; A0A1P8BEG1; -.
DR SMR; A0A1P8BEG1; -.
DR ProteomicsDB; 205782; -.
DR EnsemblPlants; AT5G42390.2; AT5G42390.2; AT5G42390.
DR GeneID; 834245; -.
DR Gramene; AT5G42390.2; AT5G42390.2; AT5G42390.
DR Araport; AT5G42390; -.
DR TAIR; AT5G42390; SPP.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; A0A1P8BEG1; baseline and differential.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 3.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR007863; Peptidase_M16_C.
DR PANTHER; PTHR43690; NARDILYSIN; 1.
DR PANTHER; PTHR43690:SF33; STROMAL PROCESSING PEPTIDASE, CHLOROPLASTIC; 1.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 2.
DR SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 2.
PE 1: Evidence at protein level;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Proteomics identification {ECO:0007829|PeptideAtlas:A0A1P8BEG1,
KW ECO:0007829|ProteomicsDB:A0A1P8BEG1};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 216..342
FT /note="Peptidase M16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00675"
FT DOMAIN 361..598
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
FT DOMAIN 928..1020
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
SQ SEQUENCE 1086 AA; 120723 MW; CCD209842BD9A83D CRC64;
MASSSSSIFT GVKFSPILAP FNSGDSRRSR YLKDSRNKVR FNPSSPRLTP HRVRVEAPSL
IPYNGLWAAQ PNSHKGRLKR NIVSGKEATG ISLSQGRNFC LTCKRNQAGI RRALPSAFVD
RTAFSLSRSS LTSSLRKHSQ IVNATLGPDE PHAAGTAWPD GIVAERQDLD LLPPEIDSAE
LEAFLGCELP SHPKLHRGQL KNGLRYLILP NKVPPNRFEA HMEVHVGSID EEEDEQGIAH
MIEHVAFLGS KKREKLLGTG ARSNAYTDFH HTVFHIHSPT HTKDSEDDLF PSVLDALNEI
AFHPKFLSSR VEKERRAILS ELQMMNTIEY RVDCQLLQHL HSENKLGRRF PIGLEEQIKK
WDVDKIRKFH ERWYFPANAT LYIVGDIDNI PRIVHNIEAV FGKNGLDNES TPSSPSPGAF
GAMANFLVPK LPAGLGGTFS NEKTNTADQS KMIKRERHAI RPPVEHNWSL PGTSVDLKPP
QIFKHELLQN FAINMFCKIP VSKVQTFGDL RNVLMKRIFL SALHFRINTR YKSSNPPFTS
VELDHSDSGR EGCTVTTLTV TAEPQNWQNA VKVAVQEVRR LKEFGVTRGE LTRYMDALLK
DSEHLAAMID NVSSVDNLDF IMESDALSHT VMDQTQGHET LVAVAGTVTL EEVNTVGAKV
LEFISDFGRP TAPLPAAIVA CVPTKVHVDG VGESDFNISP DEIIESVKSG LLAPIEAEPE
LEVPKELISQ SQLKELTLQR NPCFVPIPGS GLTKLHDKET GITQLRLSNG IAVNYKKSTT
ESRAGVMRLI VGGGRAAETS DSKGAVVVGV RTLSEGGRVG DFSREQVELF CVNHLINCSL
ESTEEFIAME FRFTLRDNGM QAAFQLLHMV LERSVWLEDA FDRARQLYLS YFRSIPKSLE
RATAHKLMIA MLNGDERFVE PTPKSLQSLN LESVKDAVMS HFVGDNMEVS IVGDFSEEEI
ERCILDYLGT VKASHDSAKP PGSEPILFRQ PTAGLQFQQV FLKDTDERAC AYIAGPAPNR
WGFTVDGDDL FQSVSKLPVA HDGLLKSEEQ LLEGGDRELQ KKLRAHPLFF GVTMGLLAEI
INSRIC
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