ID A0A1P8KDU1_9BURK Unreviewed; 535 AA.
AC A0A1P8KDU1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN ORFNames=RS694_17825 {ECO:0000313|EMBL:APW44203.1};
OS Rhodoferax saidenbachensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Rhodoferax.
OX NCBI_TaxID=1484693 {ECO:0000313|EMBL:APW44203.1, ECO:0000313|Proteomes:UP000186110};
RN [1] {ECO:0000313|EMBL:APW44203.1, ECO:0000313|Proteomes:UP000186110}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22694 {ECO:0000313|EMBL:APW44203.1,
RC ECO:0000313|Proteomes:UP000186110};
RA Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
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DR EMBL; CP019239; APW44203.1; -; Genomic_DNA.
DR RefSeq; WP_029705305.1; NZ_CP019239.1.
DR AlphaFoldDB; A0A1P8KDU1; -.
DR STRING; 1484693.RS694_17825; -.
DR KEGG; rsb:RS694_17825; -.
DR eggNOG; COG1020; Bacteria.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000186110; Chromosome.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:APW44203.1};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000186110};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:APW44203.1}.
FT DOMAIN 62..340
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 383..524
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 535 AA; 57807 MW; 3A6F2F796B5BBE2E CRC64;
MVTRVIAKKA VQKARQRVAA TVAPVAPAAR TATRTARKAA KVVQKNVTDA VVGTLGLHGE
RMSKVDTAWL RMDSASNLMM IVGVWVIQPG IRYEDLAQRL EERLLKYPRF VQCAVEDAAG
ATWVNDPNFD MGHHLVTETL PKKPAGHEQE ALQDRLAALC MEPLDPNRPL WQIHLVQNYK
GGSAMMVRIH HCIADGIALI SVTQSLVDGG APPPERAPRP ARREGWEGAE DWLYSALYKP
MTRASVKAME LASGSALRSM DMMLNPQAGM EKGLEGSLDM AKLAYQAVSD LTALALMPDD
SPTRLKGIPG NTKRVAWCAP IPLAEVKAVG KALNCSVNDV LLSCVAGAIG EYLKDHGDSV
KGVEIRAMVP VNLRPMDKAY KLGNQFGLAP LVLPIGMENP IERIYEVRTR MHGLKGSMQP
LLAFGMLAVA GLLVRPAQDA LLNLFSKKTT AVMTNVPGPA TKLKICGSTI EETLFWVPQS
GTVGLGVSIL SYGGGVQFGV VSDATLCPDP QAIIDQFEPE FAKLAMVTLM LPWGE
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