ID A0A1P8NHZ5_9MICO Unreviewed; 372 AA.
AC A0A1P8NHZ5;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=glucarate dehydratase {ECO:0000256|ARBA:ARBA00011973};
DE EC=4.2.1.40 {ECO:0000256|ARBA:ARBA00011973};
GN ORFNames=BH708_15910 {ECO:0000313|EMBL:APX33946.1};
OS Brachybacterium sp. P6-10-X1.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermabacteraceae;
OC Brachybacterium.
OX NCBI_TaxID=1903186 {ECO:0000313|EMBL:APX33946.1, ECO:0000313|Proteomes:UP000185991};
RN [1] {ECO:0000313|EMBL:APX33946.1, ECO:0000313|Proteomes:UP000185991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6-10-X1 {ECO:0000313|EMBL:APX33946.1,
RC ECO:0000313|Proteomes:UP000185991};
RA Zhao B., Liao L., Chen B.;
RT "Complete genome of Brachybacterium sp. P6-10-X1.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O;
CC Xref=Rhea:RHEA:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:30612,
CC ChEBI:CHEBI:42819; EC=4.2.1.40;
CC Evidence={ECO:0000256|ARBA:ARBA00001426};
CC -!- PATHWAY: Carbohydrate acid metabolism; D-glucarate degradation; 2,5-
CC dioxopentanoate from D-glucarate: step 1/2.
CC {ECO:0000256|ARBA:ARBA00005183}.
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DR EMBL; CP017297; APX33946.1; -; Genomic_DNA.
DR RefSeq; WP_076810031.1; NZ_CP017297.1.
DR AlphaFoldDB; A0A1P8NHZ5; -.
DR STRING; 1903186.BH708_15910; -.
DR KEGG; brx:BH708_15910; -.
DR OrthoDB; 9802699at2; -.
DR Proteomes; UP000185991; Chromosome.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR PANTHER; PTHR48080:SF4; GLUCARATE DEHYDRATASE; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SFLD; SFLDG00055; glucarate_dehydratase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Isomerase {ECO:0000313|EMBL:APX33946.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 143..237
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
SQ SEQUENCE 372 AA; 40294 MW; C21975FEF8D8DCD5 CRC64;
MKIVDLEVSV VNLPMRKGFT SSFETKQGET RTIVRIRTDD GLVGWGETMW GAPVARLVRQ
LAEDVIGTDP RALESFHARH RMLPFFHGYL GYAALAAIDV ACWDLLGKAA GMGVVDLLGG
RVREQIPLTA LITRSDGDER DYPGSLADAT EQIVAEHGFT AVKLKGSTDA RADVAIMQAL
RERLPEVELR VDPNAAWSVP ESIRAGHALE PLGLEYLEDP CQGLEQMARV AEAVRIPLCT
NMCVVRWEDL APAVRLNAVQ VIHGDVYKWG GIATTKHLAA HCDAFGLGMN LHSGGELGIA
TAAHLAIVAS TPVLSSAIDS MYYLHDDDII EPLPFEGGRL RVPDGVGLGV EVDEDKLAHY
TALNAREGDY TG
//
