ID   A0A1Q2CG86_9ACTN        Unreviewed;       558 AA.
AC   A0A1Q2CG86;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   SubName: Full=Protease-4 {ECO:0000313|EMBL:SDY55612.1};
GN   ORFNames=RPIT_10270 {ECO:0000313|EMBL:AQP45129.1}, SAMN05428934_102251
GN   {ECO:0000313|EMBL:SDY55612.1};
OS   Tessaracoccus flavus.
OC   Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC   Propionibacteriaceae; Tessaracoccus.
OX   NCBI_TaxID=1610493 {ECO:0000313|EMBL:AQP45129.1, ECO:0000313|Proteomes:UP000188324};
RN   [1] {ECO:0000313|EMBL:AQP45129.1, ECO:0000313|Proteomes:UP000188324}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RP1T {ECO:0000313|EMBL:AQP45129.1,
RC   ECO:0000313|Proteomes:UP000188324};
RX   PubMed=26869509; DOI=10.1099/ijsem.0.000958;
RA   Kumari R., Singh P., Schumann P., Lal R.;
RT   "Tessaracoccus flavus sp. nov., isolated from the drainage system of a
RT   lindane-producing factory.";
RL   Int. J. Syst. Evol. Microbiol. 66:1862-1868(2016).
RN   [2] {ECO:0000313|EMBL:SDY55612.1, ECO:0000313|Proteomes:UP000199635}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RP1 {ECO:0000313|EMBL:SDY55612.1,
RC   ECO:0000313|Proteomes:UP000199635};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:AQP45129.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=RP1T {ECO:0000313|EMBL:AQP45129.1};
RA   Peterson S.W.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family.
CC       {ECO:0000256|ARBA:ARBA00008683}.
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DR   EMBL; CP019605; AQP45129.1; -; Genomic_DNA.
DR   EMBL; FNPU01000002; SDY55612.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Q2CG86; -.
DR   STRING; 1610493.RPIT_10270; -.
DR   KEGG; tfl:RPIT_10270; -.
DR   OrthoDB; 9764363at2; -.
DR   Proteomes; UP000188324; Chromosome.
DR   Proteomes; UP000199635; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR   CDD; cd07018; S49_SppA_67K_type; 1.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR004634; Pept_S49_pIV.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR047217; S49_SppA_67K_type_N.
DR   InterPro; IPR047272; S49_SppA_C.
DR   PANTHER; PTHR33209; PROTEASE 4; 1.
DR   PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR   Pfam; PF01343; Peptidase_S49; 2.
DR   PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR   SUPFAM; SSF52096; ClpP/crotonase; 2.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:SDY55612.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000188324};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825}.
FT   DOMAIN          110..239
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   DOMAIN          342..493
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   ACT_SITE        161
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT   ACT_SITE        359
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ   SEQUENCE   558 AA;  59523 MW;  7DCE005A7E848A48 CRC64;
     MGLKDLLGKI TGDTPVVLEL DMARGVLVAK PTNPLQAVQL INSPTLNALR SHLREASRDA
     RVLGLIVHAV PSGTALAYVE EIAGLIEDFG ESKKTMAWSE SFGELGQGLG AYRLAAAAQQ
     IWLQPTGAVN IEGVEVSMTL LKGLFNKAGA EPQFGQRHEY KTAADTYAAD HVTEANREMT
     SRLGQSIVEE FVAAVARLRS IPPERVWDGV NGSPLTAEQA LELGLIDRVG YRDEAYAATL
     EEWGAEADHL LFAHRYTNKA AMAKKLAPKQ PKVAVVTLRG AIVTGRGQRS PMGGDQSGSD
     VVDEHLRAAL RDEDVKAVVF EVDSPGGSAV ASDFIRRSVL RLREAGKPVV AHMGAVAASG
     GYYVAMPCDE IVAEPSTLTG SIGVLAGKVV TRGTYDKLGL VRETIPVGAA AGMLSSAHEF
     SDDDWDRLNR WLDRVYLDFT TFAAQDRGMD HDHLESLARG RVWTGADARD RGLVDHLGGQ
     WLAVERACAL AGLDERRTKL THIGDAGLLT LLKPATSTEG AVGGANLPDA EGVLTALAAR
     VGLHGRGALT MPWGLRIS
//