ID A0A1Q2D649_9ENTE Unreviewed; 732 AA.
AC A0A1Q2D649;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=BW732_05690 {ECO:0000313|EMBL:AQP53781.1}, CBF34_08340
GN {ECO:0000313|EMBL:RSU00388.1};
OS Vagococcus penaei.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX NCBI_TaxID=633807 {ECO:0000313|EMBL:AQP53781.1, ECO:0000313|Proteomes:UP000188246};
RN [1] {ECO:0000313|EMBL:AQP53781.1, ECO:0000313|Proteomes:UP000188246}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CD276 {ECO:0000313|EMBL:AQP53781.1,
RC ECO:0000313|Proteomes:UP000188246};
RX PubMed=19897618; DOI=10.1099/ijs.0.012872-0;
RA Jaffres E., Prevost H., Rossero A., Joffraud J.J., Dousset X.;
RT "Vagococcus penaei sp. nov., isolated from spoilage microbiota of cooked
RT shrimp (Penaeus vannamei).";
RL Int. J. Syst. Evol. Microbiol. 60:2159-2164(2010).
RN [2] {ECO:0000313|EMBL:AQP53781.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CD276 {ECO:0000313|EMBL:AQP53781.1};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:RSU00388.1, ECO:0000313|Proteomes:UP000286995}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 24833 {ECO:0000313|EMBL:RSU00388.1,
RC ECO:0000313|Proteomes:UP000286995};
RA Gulvik C.A.;
RT "Vagococcus spp. assemblies.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
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DR EMBL; CP019609; AQP53781.1; -; Genomic_DNA.
DR EMBL; NGJV01000016; RSU00388.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q2D649; -.
DR STRING; 633807.BW732_05690; -.
DR KEGG; vpi:BW732_05690; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000188246; Chromosome.
DR Proteomes; UP000286995; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000188246}.
FT DOMAIN 31..287
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 652..728
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 481
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 551
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 732 AA; 83485 MW; E2A62270F4BC842C CRC64;
MEKLILVNQE QTYFHLTNGR ISYLLEIEDY GLVAQVYFGQ AIREYHGQRK YPRIDRGFSG
NLPGCLERGY SPDTLPRDYS FSGDGDYRTP AFIGYHADGS RLTHLTFSKY DIMNGKPKLV
GLPQAYVLDE TEAQTLILTL EDSVSQLVVQ LFYTIYRDRD VVTRSVKVLN HGQSAIELHK
VASLQLDLPS ADYELLSFHG AHVNERQLQC ESIAYGCKRL ASRRGTTSHH MTNSIMLATP
QTTENQGEVY GFNLVYSGNF AIDIERGQVN QLRITIGIND ENFNWLLSPS ETFQSPEAVM
TFSNQGLNGM SRTFHQFIRE RIVRGVHQYR ERPILVNNWE ATYFDFTESI LRPIVDEAAA
LGIEMFVLDD GWFGQRDDDT TSLGDWQVNA RKFPNGLKAF ADYVHEKGLQ FGLWVEPEMI
SFASKLYQTH PDYVLALPNR EPSPSRSQYV LDLSRDEVQE NVYQQLKSLL DEGFIDYLKW
DMNRHLSDVF SYALPKERQG EVAHRYTLGL YALLERLTTE YPNILFEGCS GGGGRLDTGL
AYYMPQSWTS DNTDAVDRLK IQYGTSYIYP PATFTSHVSA VPNHQTGRTT SLEMRGNVAM
SSVFGYELDL TTLTESEKLS VKQQVATYKT IRRLVQFGDF IRLASPFDGN TCAWEFVTPD
RSECLVFTFQ VLSSAQPTIS LIKLAGLDPK REYQNLATNE IVGGDELMSL GFYQVSRQED
FVSWRYHFKA ID
//
