ID A0A1Q3DW70_LENED Unreviewed; 701 AA.
AC A0A1Q3DW70;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Non-heme halogenase {ECO:0000313|EMBL:GAV99189.1};
GN ORFNames=LENED_000628 {ECO:0000313|EMBL:GAV99189.1};
OS Lentinula edodes (Shiitake mushroom) (Lentinus edodes).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Marasmiineae; Omphalotaceae; Lentinula.
OX NCBI_TaxID=5353 {ECO:0000313|EMBL:GAV99189.1, ECO:0000313|Proteomes:UP000188533};
RN [1] {ECO:0000313|EMBL:GAV99189.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAV99189.1,
RC ECO:0000313|Proteomes:UP000188533};
RG Lentinula edodes genome sequencing consortium;
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAV99189.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAV99189.1,
RC ECO:0000313|Proteomes:UP000188533};
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RT "A genome survey and senescence transcriptome analysis in Lentinula
RT edodes.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride + FADH2 + melleolide F + O2 = 6'-chloromelleolide F +
CC FAD + H(+) + 2 H2O; Xref=Rhea:RHEA:67160, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:57692, ChEBI:CHEBI:58307, ChEBI:CHEBI:167712,
CC ChEBI:CHEBI:167713; Evidence={ECO:0000256|ARBA:ARBA00036433};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67161;
CC Evidence={ECO:0000256|ARBA:ARBA00036433};
CC -!- SIMILARITY: Belongs to the flavin-dependent halogenase family.
CC {ECO:0000256|ARBA:ARBA00005706}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAV99189.1}.
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DR EMBL; BDGU01000012; GAV99189.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q3DW70; -.
DR STRING; 5353.A0A1Q3DW70; -.
DR Proteomes; UP000188533; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0140907; F:flavin-dependent halogenase activity; IEA:UniProt.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR006905; Flavin_halogenase.
DR PANTHER; PTHR43747:SF1; FAD-BINDING DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43747; FAD-BINDING PROTEIN; 1.
DR Pfam; PF01494; FAD_binding_3; 1.
DR Pfam; PF04820; Trp_halogenase; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 603..625
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 15..141
FT /note="FAD-binding"
FT /evidence="ECO:0000259|Pfam:PF01494"
FT REGION 222..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..241
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 701 AA; 77659 MW; ACFBD332988CA9D3 CRC64;
MYETRQDLAV PTKHVEVLII GGGPAGSYAA SVLSREGIDV AVLESTKFPR YHIGSLIPSV
RPYLRFIGAE EKIVQHGFTK KPGSAIKFNQ FKREGYTDFV ALGADNNAWN VIRSEFDHLL
LNHARECGAK VYEETKVTNI AFDPIDPEKP VSVSWTRTCT AQSPNASQNG TTKAVHGSTT
FTHLIDASGR AGLLSTRYLK NRHFNASLKN IAVWGYWHNR SDEEPDNEHD KEDHKPRVGM
YGKGTSREGA PWFEALTDES GWAWFIPLHN DKTSVGIVMN QEQYNKSRPS SSDAQYECSL
VGRYLANLRL APGVARLLCG KDVVHNGSDS EHEIQIPIEE EILHQQAKLE PGSVRSASDF
SYSAPSYAGK GFRIVGDAGA FIDPFFSSGI HLAFTGALSA AATICASRRR NCTEVEAAMW
HTKRVAVSYT RFQVVVLSAY KQMRAQSIDV LSDVDEDNFD RAFTYLRPVI QGASDMGSRL
SESELQRSLD FCINLFNPTS PEEHKEVEGR LAGLGRYREF MDVAKPLVDP KSFEDVLKVT
PTECSARSTG CKKTEANTYS ESKPRMVLDK INARRLVHPE YAINNLEAEE LLGLREDSHE
IQVIMSFLLG PVSGALVAGG IYYGFSNLIQ ARTKQLSTDL HIQSTLLLHS PTPAWISTYP
SPAASRIEHT FVGVGQADKH SELRVLEYRF ATNPSCGTQV G
//
