ID A0A1Q3DX20_LENED Unreviewed; 710 AA.
AC A0A1Q3DX20;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=General negative regulator of transcription subunit {ECO:0000256|PIRNR:PIRNR005290};
GN ORFNames=LENED_000949 {ECO:0000313|EMBL:GAV99489.1};
OS Lentinula edodes (Shiitake mushroom) (Lentinus edodes).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Marasmiineae; Omphalotaceae; Lentinula.
OX NCBI_TaxID=5353 {ECO:0000313|EMBL:GAV99489.1, ECO:0000313|Proteomes:UP000188533};
RN [1] {ECO:0000313|EMBL:GAV99489.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAV99489.1,
RC ECO:0000313|Proteomes:UP000188533};
RG Lentinula edodes genome sequencing consortium;
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAV99489.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAV99489.1,
RC ECO:0000313|Proteomes:UP000188533};
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RT "A genome survey and senescence transcriptome analysis in Lentinula
RT edodes.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery.
CC {ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005290}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family.
CC {ECO:0000256|ARBA:ARBA00007682, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAV99489.1}.
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DR EMBL; BDGU01000014; GAV99489.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q3DX20; -.
DR STRING; 5353.A0A1Q3DX20; -.
DR Proteomes; UP000188533; Unassembled WGS sequence.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IEA:UniProtKB-UniRule.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 2.30.30.1020; CCR4-NOT complex subunit 2/3/5, C-terminal domain; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_C_sf.
DR InterPro; IPR012270; CCR4-NOT_su3/5.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; CCR4 NOT-RELATED; 1.
DR PANTHER; PTHR23326:SF1; CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 3; 1.
DR Pfam; PF04153; NOT2_3_5_C; 1.
DR Pfam; PF04065; Not3; 1.
DR PIRSF; PIRSF005290; NOT_su_3_5; 2.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|PIRNR:PIRNR005290};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR005290};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR005290};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|PIRNR:PIRNR005290};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR005290};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|PIRNR:PIRNR005290}.
FT DOMAIN 3..231
FT /note="CCR4-Not complex component Not N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04065"
FT DOMAIN 582..707
FT /note="NOT2/NOT3/NOT5 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04153"
FT REGION 145..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..510
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..400
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..499
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 710 AA; 78786 MW; FF0DC9A952E824AC CRC64;
MAARKLQTEI DRTLKKVGEG VEYFESVYDK MQASSNQTQK EKLELDLKTQ IKKLQRMRDQ
IKTWYASNDI KDKSALVENR KLIETQMEKF KACEKEMKTK AFSKEGLTQA AKLDPKEQEK
EEIMAWLQTK VEELQMQIEQ AEAEIESLQG SGKKRGKSSS TAGRAEELEH LNDRRKWHIS
RLEIVLRLLN NGSLFTEKAV ALKDDVAYFV ESNTEEDFDE YEGIYDELNL DEEEEKFRGL
VHDDEDSDES DDASEDLPPR TPNKKHDDES TTSSRHGSSP ILKKATTTLR KGSVDAKAQP
ITNILKTTVP HPAPVLAAPP PRPAVTLPPI RYAAAAAAAI APVPTPPTKA AASTNSGPNA
TPAFSIPTSS SQASQDQVSV VPSSPSLTHP SVTSPMLSSA ASVSQQFDGS FYSSQESPAM
SEAVISSVGA PVATSSPHRA SIARKDSISS PSKAANTTMD APPPSQSQTN GALQASALNP
SPLPQQLQQY SGVPAPSSPA PQSAQAPQFP PGVKVAVNEQ SLLVPQQNVP TARPPSVAQQ
APRAVPGSLS DLVVSFENAK QKAVHRMNNL DQVHKLLEGG LSNLPQPQDT ERPKYYVPRN
PFNTPSYYPQ MPNPLLNTPA IFAQLDVETL FYVFYYLPGT YQQFLAAKEL KRQSWRFHVK
YLTWFQRHSE PQAITEEYEQ GVYVYFDWEG SWCQRKKADF RFEYRYLSED
//
