ID A0A1Q4H6F1_9MYCO Unreviewed; 519 AA.
AC A0A1Q4H6F1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Methionine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_01228};
DE EC=6.1.1.10 {ECO:0000256|HAMAP-Rule:MF_01228};
DE AltName: Full=Methionyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_01228};
DE Short=MetRS {ECO:0000256|HAMAP-Rule:MF_01228};
GN Name=metG {ECO:0000256|HAMAP-Rule:MF_01228};
GN ORFNames=BRW64_23525 {ECO:0000313|EMBL:OJZ63118.1}, BV510_30745
GN {ECO:0000313|EMBL:OPE44673.1}, CRI78_17635
GN {ECO:0000313|EMBL:PEG53204.1};
OS Mycolicibacterium diernhoferi.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1801 {ECO:0000313|EMBL:OJZ63118.1, ECO:0000313|Proteomes:UP000186837};
RN [1] {ECO:0000313|EMBL:OPE44673.1, ECO:0000313|Proteomes:UP000191039}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BM1 {ECO:0000313|EMBL:OPE44673.1,
RC ECO:0000313|Proteomes:UP000191039};
RA Greninger A.L., Jerome K.R., Mcnair B., Wallis C., Fang F.;
RT "genome sequences of unsequenced Mycobacteria.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OJZ63118.1, ECO:0000313|Proteomes:UP000186837}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bard {ECO:0000313|EMBL:OJZ63118.1,
RC ECO:0000313|Proteomes:UP000186837};
RA Greninger A.L., Fang F., Jerome K.R.;
RT "Genome sequences of unsequenced Mycobacteria.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:PEG53204.1, ECO:0000313|Proteomes:UP000220340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IP141170001 {ECO:0000313|EMBL:PEG53204.1,
RC ECO:0000313|Proteomes:UP000220340};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Is required not only for elongation of protein synthesis but
CC also for the initiation of all mRNA translation through initiator
CC tRNA(fMet) aminoacylation. {ECO:0000256|ARBA:ARBA00003314,
CC ECO:0000256|HAMAP-Rule:MF_01228}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01228};
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01228}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01228}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC MetG type 2B subfamily. {ECO:0000256|HAMAP-Rule:MF_01228}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_01228}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OJZ63118.1}.
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DR EMBL; MPNS01000018; OJZ63118.1; -; Genomic_DNA.
DR EMBL; MIJD01000684; OPE44673.1; -; Genomic_DNA.
DR EMBL; PDCR01000022; PEG53204.1; -; Genomic_DNA.
DR RefSeq; WP_073858882.1; NZ_PDCR01000022.1.
DR AlphaFoldDB; A0A1Q4H6F1; -.
DR STRING; 1801.BRW64_23525; -.
DR OrthoDB; 9810191at2; -.
DR Proteomes; UP000186837; Unassembled WGS sequence.
DR Proteomes; UP000191039; Unassembled WGS sequence.
DR Proteomes; UP000220340; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 2.170.220.10; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01228; Met_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_01228};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_01228};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01228};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_01228};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_01228};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_01228}.
FT DOMAIN 13..372
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 384..515
FT /note="Methionyl-tRNA synthetase anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF19303"
FT MOTIF 20..30
FT /note="'HIGH' region"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01228"
FT MOTIF 309..313
FT /note="'KMSKS' region"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01228"
SQ SEQUENCE 519 AA; 57947 MW; 288BFC2621F5A282 CRC64;
MTESSVSAAP RAFYVTTAIA YPNGDPHIGH AYEYIATDAI ARFKRLDGYD VRYLTGTDVH
GLKMAETAAK LGITAAELAK RNSDVFERMQ ERLDASYDRF IRTSDADHYA ASKEIWRRMN
EAGDIYLGSY QGWYSVRDER FFAEDETEVA PDGTRTAIET GTEVTWTEEQ TYFFKLSEYT
DRLLAHYEAH PEFIGPDVRR NEVVSFVSGG LRDLSISRTT FDWGVPVPDH PDHVMYVWVD
ALTNYLTGVG FPDTASESFE KYWPADLHMI GKDIIRFHTV YWPAFLMSAG IPLPRRVFAH
GFINVKGEKM SKSLGNVVDP LVLVDTFGVD AVRYFFLREV PFGQDGSYSE EAIIGRVNAD
LANELGNLAQ RSLSMVAKNL DGVVPEPGPF TAEDEALLAA ADALLERVRA CYDEQAMHLA
LDAIWSVLSA ANKYFSAQEP WVLRKTDPAR MATVLYTTLE TVRIATLLSQ PVMPSSTAKL
LDLLGQPADR RDFTAIGARL SAGTALPTPE GVFPRYQTE
//
