ID A0A1Q4HGP5_9MYCO Unreviewed; 400 AA.
AC A0A1Q4HGP5;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Uroporphyrinogen-III C-methyltransferase {ECO:0000313|EMBL:OJZ66683.1};
GN Name=cobA {ECO:0000313|EMBL:PEG56561.1};
GN ORFNames=BRW64_10635 {ECO:0000313|EMBL:OJZ66683.1}, CRI78_01575
GN {ECO:0000313|EMBL:PEG56561.1};
OS Mycolicibacterium diernhoferi.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1801 {ECO:0000313|EMBL:OJZ66683.1, ECO:0000313|Proteomes:UP000186837};
RN [1] {ECO:0000313|EMBL:OJZ66683.1, ECO:0000313|Proteomes:UP000186837}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bard {ECO:0000313|EMBL:OJZ66683.1,
RC ECO:0000313|Proteomes:UP000186837};
RA Greninger A.L., Fang F., Jerome K.R.;
RT "Genome sequences of unsequenced Mycobacteria.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PEG56561.1, ECO:0000313|Proteomes:UP000220340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IP141170001 {ECO:0000313|EMBL:PEG56561.1,
RC ECO:0000313|Proteomes:UP000220340};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00005879}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OJZ66683.1}.
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DR EMBL; MPNS01000004; OJZ66683.1; -; Genomic_DNA.
DR EMBL; PDCR01000001; PEG56561.1; -; Genomic_DNA.
DR RefSeq; WP_073856162.1; NZ_PDCR01000001.1.
DR AlphaFoldDB; A0A1Q4HGP5; -.
DR STRING; 1801.BRW64_10635; -.
DR OrthoDB; 9815856at2; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000186837; Unassembled WGS sequence.
DR Proteomes; UP000220340; Unassembled WGS sequence.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:InterPro.
DR GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd11642; SUMT; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR006366; CobA/CysG_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR012409; Sirohaem_synth.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR NCBIfam; TIGR01469; cobA_cysG_Cterm; 1.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR45790:SF3; S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF00590; TP_methylase; 1.
DR PIRSF; PIRSF036426; Sirohaem_synth; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis {ECO:0000256|ARBA:ARBA00022573};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:OJZ66683.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OJZ66683.1}.
FT DOMAIN 164..375
FT /note="Tetrapyrrole methylase"
FT /evidence="ECO:0000259|Pfam:PF00590"
FT ACT_SITE 194
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036426-1"
FT ACT_SITE 216
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036426-1"
SQ SEQUENCE 400 AA; 42062 MW; 9840F875600E0163 CRC64;
MTENAYLVGL RLEGRKVVVV GGGTVAQRRL PLLIAAGADV HVIAREATPA VEAFQPITLA
LRDFRDEDLD GAWYVLAATD DPDVNAAIVA GAERRRIFCV RADVAREGTA VTPATFDYEG
LSVGVLAGGE HRRSAAIRSA IRDALQRGLI TDRGSDDDVV REGVALVGGG PGDPELITVR
GRRLLAQADV VVADRLAPPE LLAELAPHVE VIDAAKIPYG RAMAQEAINN VLIERAKEGK
FVVRLKGGDP FVFARGYEEV IACAEAGVPV TVVPGVTSAI AVPALAGIPV THRHVTHEFV
VVSGHVAPDH PESLVNWDAL AAMSGTIVLL MAVERIELFA QALLAGGRPA DTPVMVVQHG
TTAAQRTLRA TLSDVGERVR SEGIRPPAII VIGAVAGFGN
//
