ID A0A1Q4UPB1_9PROT Unreviewed; 940 AA.
AC A0A1Q4UPB1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=LF95_11560 {ECO:0000313|EMBL:OKH87441.1};
OS Thalassospira sp. TSL5-1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Thalassospiraceae; Thalassospira.
OX NCBI_TaxID=1544451 {ECO:0000313|EMBL:OKH87441.1, ECO:0000313|Proteomes:UP000186302};
RN [1] {ECO:0000313|EMBL:OKH87441.1, ECO:0000313|Proteomes:UP000186302}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TSL5-1 {ECO:0000313|EMBL:OKH87441.1,
RC ECO:0000313|Proteomes:UP000186302};
RA Zhou H., Wang H.;
RT "Genome sequencing of Thalassospira sp. TSL5-1.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKH87441.1}.
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DR EMBL; JRJD01000003; OKH87441.1; -; Genomic_DNA.
DR RefSeq; WP_073955165.1; NZ_KV880638.1.
DR AlphaFoldDB; A0A1Q4UPB1; -.
DR STRING; 1544451.LF95_11560; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000186302; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 9..264
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 317..529
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 698..904
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 940 AA; 103141 MW; 31427865E6FAE68D CRC64;
MTDTAQPPRR VVLVDGSGFI FRAFHALPPM NSPDGTPVNA VYGFCTMLMK LREDTKPDHM
AVIFDTKGKS FRNDFYPDYK AHRPPPPEEL VPQFGLIRDA VRAFNLPSIE LEGYEADDLI
ATYARQAAEQ GADVIIVSSD KDLMQLVTER VEMFDAMKNR TIRSGEVMEK FGVGPDKVID
VQALAGDPVD NVPGVPGIGV KTAAQLITEY GDLDSLLERA GEIKQPKRRE KLIENADMAR
ISRDLVRLKT DVPVTHPVDD FDLREPDADV LLSFIATHGF KSLTAKVKAK FGGTVDENGV
AEAGTGVNDV TVTKAEYELV QDEESLKKWI AKADYEGILA VDTETTSLNA HQARLVGISI
AVQPGEACYI PLTHGMGDEA TEPKAAQGGF DFGSDPADDK AKAEIPQQIP LKRAIELLKP
LLEDRGVLKI GQNIKYDKII FARHGIDVAP IDDTMVLSYV LDGTSHGHGM DELADLHLGY
KTIKFTDVAG TGKNQLTFDQ VPLDKALDYA AEDADITLRL HRLLKPRIAQ EHMASVYETL
DRPLVPVLAK MEALGVKVDA DKLRELSSDF ASRMEVLEKT IHEMAGETFN LGSPKQLGEI
LFERMSLPGG KKTKTGAWQT GADVLEGLAA QGHDLPARIL DWRQLSKLKS TYTDALANHI
NPQTGRVHTS YGQTNVNTGR LSSNDPNLQN IPIRSEEGRK IRTAFIADEG CKLISADYSQ
IELRLLAHVA EIDALRTAFK NGEDIHASTA SKVFGVPIEG MDPMVRRKAK AINFGIIYGI
SAFGLANQLG IPQKEAKEFI NAYFEIYPGI RDYMERAKDF AKTHGMVRTL FGRKIFINGI
NDKNGMRRSF GERAAINAPI QGGAADIIKR AMIAVPDALL DAGLKTRMLL QVHDELIFEA
PEAETEQAVK VIRDVMENAA HLSVPLIADA GIGDSWADAH
//
