ID A0A1Q4VTS0_9ACTN Unreviewed; 378 AA.
AC A0A1Q4VTS0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369};
GN ORFNames=A6A06_20645 {ECO:0000313|EMBL:OKI01222.1};
OS Streptomyces sp. CB02923.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1718985 {ECO:0000313|EMBL:OKI01222.1, ECO:0000313|Proteomes:UP000185893};
RN [1] {ECO:0000313|EMBL:OKI01222.1, ECO:0000313|Proteomes:UP000185893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB02923 {ECO:0000313|EMBL:OKI01222.1,
RC ECO:0000313|Proteomes:UP000185893};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC {ECO:0000256|ARBA:ARBA00005163}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKI01222.1}.
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DR EMBL; LWKZ01000024; OKI01222.1; -; Genomic_DNA.
DR RefSeq; WP_073764309.1; NZ_LWKZ01000024.1.
DR AlphaFoldDB; A0A1Q4VTS0; -.
DR STRING; 1718985.A6A06_20645; -.
DR OrthoDB; 9800864at2; -.
DR UniPathway; UPA00223; -.
DR Proteomes; UP000185893; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR011278; 2-MeCitrate/Citrate_synth_II.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR NCBIfam; TIGR01800; cit_synth_II; 1.
DR PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR11739:SF4; CITRATE SYNTHASE, PEROXISOMAL; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000185893};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369}.
FT ACT_SITE 263
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 317
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 378 AA; 41235 MW; F4C2FABC5E68A2E7 CRC64;
MTTTPPEIHR GLAGVVVDTT AVSTVITETN SLTYRGYPVQ ELAAHRSFEE VAYLLWYGDL
PDAAELREFQ ARERALRPLD RTTSDLLARL PQSCHPMDVL RTAVSFFGAE DPAEDDGSTA
ANRAKSLSLL AKLPVVVAAE HRRRRGLAPV LPDPGLGFAE NFFHMCFGRV PDPAVVRCFE
ISLILYAEHS FNASTFTARV VTSTLSDLYS AVTAAIGALK GPLHGGANEA VMHMLEEIGA
PERADAWLTD AFADQRKIMG FGHRVYKHGD SRVPVMQEAT DRLVALADTP EAARLAALHT
ALREAVLRRK GIHPNLDYPA GLAYHLMGFD IPAFTPIFVM SRITGWTAHI TEQLAHNALI
RPLGAYNGPG QRGVPEVV
//
