ID A0A1Q4W632_9ACTN Unreviewed; 4290 AA.
AC A0A1Q4W632;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Dimodular nonribosomal peptide synthetase {ECO:0000313|EMBL:OKI05542.1};
GN ORFNames=AMK13_19435 {ECO:0000313|EMBL:OKI05542.1};
OS Streptomyces sp. CB02056.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703924 {ECO:0000313|EMBL:OKI05542.1, ECO:0000313|Proteomes:UP000186379};
RN [1] {ECO:0000313|EMBL:OKI05542.1, ECO:0000313|Proteomes:UP000186379}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB02056 {ECO:0000313|EMBL:OKI05542.1,
RC ECO:0000313|Proteomes:UP000186379};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKI05542.1}.
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DR EMBL; LIPD01000007; OKI05542.1; -; Genomic_DNA.
DR RefSeq; WP_074003396.1; NZ_LIPD01000007.1.
DR OrthoDB; 2472181at2; -.
DR Proteomes; UP000186379; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd17652; A_NRPS_CmdD_like; 1.
DR CDD; cd12117; A_NRPS_Srf_like; 1.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd19540; LCL_NRPS-like; 3.
DR Gene3D; 3.30.300.30; -; 5.
DR Gene3D; 3.40.50.980; -; 6.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 3.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 3.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR020802; PKS_thioesterase.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR001031; Thioesterase.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF13193; AMP-binding_C; 3.
DR Pfam; PF00668; Condensation; 3.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF00550; PP-binding; 4.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00823; PKS_PP; 4.
DR SMART; SM00824; PKS_TE; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 6.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000186379};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 371..446
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1832..1907
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2895..2970
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3950..4025
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 353..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1815..1835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3190..3209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4290 AA; 460275 MW; 18A44F1F7E70B54E CRC64;
MANDRPTRPH PLSPAYVIYT SGSTGLPKGT VIPGENLVNL LTDFQERLDF GPDDRMLAVT
TFGFDIANLE LLLPLLSGGE LVLAGQDGAH DPQTLKQLIT DAGVTAVQAT PTLWRSMLDH
GPDGLTGLKV LVGGEALPEG LARELGEAAA EVTNVYGPTE TTIWSTAATV DPALPGRPPI
GRPLANTRTY VLDRALRPAP TGATGELFIA GAGVARGYLN RPGLTAERFV ADPFGPPGSR
MYRTGDLVRW RDDGSLEYLG RTDGQVKVRG FRIELGEVEH ALTDHLEVAQ AAVVVRDLGS
GGPALTGYAV PVPGTAPDPA ALRDHLARRL PGHMVPAVLT VLDVLPLTPN GKLDRKALPT
PDLGPAGPGR RPRTPQEEIL CELFAEVLEV SRVGLDDDFF QLGGHSLLAT RLVNALRSRL
RVEIPVRTLF EAPTVAALAA RIDGSAGART PLAPAARPAR IPLSFGQRRL WFINRLADDG
SAYHMQSVLR LRGELDRAAL AEALADVAAR HEALRTVFPE VDGEPHQLVL DPREARPVLT
VTDTGEADLP GRLRAAAAEA FDLTREAPLR AHLFALSPTE HVLLVVQHHI AADGWSLTVL
ARDLAQAYRS RSRGQEPGWR PLPVQYADYT LWQREVLGGP DDPASVLAVQ TGFWQDALRA
LPEQTVLPTD RPRSREAGFH GETVGLSLDA ELHQQLLDLA HRHNASLFMV LQAALAALCT
RLGAGTDIPI GSPISGRTDA ALEDLIGFFV NTLVLRTRTS GDPRFTELLT AVRDNALAAY
AHQDLPFERL VEILKPERSL SQHPLFQVFL ALQNTPDAEL RFADLDVTLA EEPLPSAKFD
LAFNLSERFR EGRLADGVQG TVEYRTDIFD RTTVETVVER LTRFLRAVAA DPRRRIGAVE
ILGEDERRRL LAGTPALVTG AEEVLHVRFS RTAAEHPDGV ALRAGERELT YRDVDRRADA
LARSLTARGV GPESRVAVFL ERSPELVVSM LAVWKAGGVC VPLHEAYPAE RLEYVMADTA
ASVLLTDRAM LAREFRHEAR VVVVGADQVP ADAAATGPDL PVVHPDQLAT IMYTSGSTGL
PKGVAVAHRD VVALADDPCW DSGNHERVLM HAPYAFDISD YELWVPLLAG NEVVLAPPGP
LDTAAIARLI ADEKITAVHF TAGLLRVVAE DAPECLATVR EVLTGGDVVS PIAVQRVLEH
APGAVVRQLY GPTEMTLCAT HHELRSPYTA DVRVPLGRPL AGTRVYVLDE WLRPVPPGVT
GEAWLAGTGL ARGYLHRPGL TAERFVADPF GAPGSRMYRT GDLARWNGSG QLEFMGRGDD
QVKIRGFRVE PGEVAAVLAD HPEVSEAEVL ARTDHRGERQ LVAYVVAGGL RPADQETGAV
QHVAEWRQIY DGMYDGARSA AFGSDFSGWE SSYDGRPIPL DEMDEWRRST VERIRELRPR
HVLEVGVGSG LILSRLYAEC ESYWATDFSE QAIRTLRAHL GAHPTVRLST QTADDLTGLP
AGHFDTIVLN SVVQYFPDAD YLRRVLTALT GLLAPGGSLF VGDVRNLDLL HTFHTGVELA
RADDAEDSSE LRRRARRRAD REKELLVAPR YFHAFARATD GLAGADVRVK RGHAHNELTR
YRYDVVLHAA VDAPVPPVEA TTLHWTQDLT DLADLQAHLA QHRPASLRVT GVPDGRTGPE
AAATAALDAG EPLARVRARL AQAVGVDPAL LYELGDRLGY RTAVTWSPSG PGGLLDVVFA
DASGPVGPLL PTGRAEPAPE DDTARDTNAP LLFQQAGRLA KALRAHVGAR LPAYMVPAAF
MVLERLPVTA NGKLDRAALP EPDREVQAGS GAPRSPREEA LCTLFADVLG LPEAGIEDDF
FQLGGHSLLA IRLVNAIRSA FGAELDVRTV FEAPTVAALA ERLDRAAPAQ APLVPMERPD
PLPVSFAQRR LWFLHRLEEH GGAYNIPIAL RLSGPVRERE LRSALRDLVV RHESLRTVFA
EVDGEPVQRI LDPAQARPGL AVVDTDADAL ERSLLAEAGR GHDLGAELPL RATLFRLTPT
DHVLLVVLHH IVGDGWSMDP LARDLGTAYA ARLADRAPEF PPLPVQYADY ALWQRAVLGS
EQDPDSRISG QIAYWRDRLA GLPEGLELPT DRPRPPEFSY RGAEVAFRLP AELHTDLLGL
AREHGATLFM VLHAALAALL TRLGAGTDLP IGTPVAGRAD ASLEGLVGFF VNTLVLRTDT
SGNPAFGDLL RRVAADDLEA YAHQDVPFER LVEVLAPPRS LARHPLFQVM LALQPGGGEA
VTLPGLDVTG QRIPQRTAKF DLDVQLRETR SPAGEPAGVE GTLGYCADLF DEASAVALAD
RLIRFLSALA ADPALPIGDV DILTPQERPR PADAQPSGPA HFDPVPLRFA AQARRTPAAP
AVSSAGRTLS YAELDAEADR LARLLVAGGA GPEHVVAVAL PRSERMLVAV LAVLKSGAAY
LPVDRAQPAE RIAFACADAR PILMLTEGEP VPGMPEPSVL PRVRLDDPAV RDALAGTPAD
ADARSAEERC AAIAADSPAY VIYTSGSTGR PKGVVVSHGS MARLLDWAAD RFGAEGLADV
LAATSLSFDV SVFELFAPLL TGGHVEIVRD LLELAERPWS GSLVSGVPSV FAALLAGGEA
AFSARTLVMA GEALPEHLVR EVRRLLPGTR LANIYGPTEA TVYATAWYSE DEEASGAESA
APPIGRPLPH VRAHVLDERL RPVPTGVRGE LYLAGPGLAH GYLRRPALTA ERFVADPFGG
PGARMYRTGD LVRRRPDGAL DYLGRTDDQV KVRGFRIEPG EIENVLTRHP AVTGAAVSAA
ADPAGDRRLV AHVALRPGNG VTGAELAAHA AATLPEYMVP TAFVLLDALP TNANGKLDRG
ALPAPDLTAR AAGGRARDPR EELMGELFAQ LLAVPSVGPD DGFFALGGHS LLATRLGNAV
RSAFGVELPL RAVFETPTPA GLVARLDTAD TARPALVPAE RPAVIPLSYA QQRLWFLNRL
DGGSGAYNIP LALRLSGELD PAALDAALRD VVERHEALRT VFPDHDGDPH QRILAAEDGP
VALVTVPVTA AELPAALSAA TRTGFDLAVE PPLRTALFRI SPTEHVLLLV LHHIAGDGSS
YAPLARDLTT AYRDRSAGRA PRWEPLAVQY ADYTLWQREL LGTEDDPDSA IARQLAHWQD
ALADLPEELT LPTDRPRPTT PGHRGGHVPL DLDARLHRKL TELSRREGVT TFMVFQAALA
ALLTRLGAGT DIPVGSPLAG RGDQALDRLV GFFANTVVLR TDTSGDPDFV TLLHRVRTTA
LAAYAHQDVP FERLVEVLSP TRSVSRHPLF QVLLVLQNTE PPRFDLDGLT ITQPETDAGT
ARFDLDVSLR ESHTPEGLTA GVQGSIRYNA DLFDEATVTA LAGRLVALLA AVADDPRAPI
GSLDVLLPPE RLDLEQWSGA PEPPVPARTM PELFQGQVAR TPDRTALRYG ADTLSYAELN
RRANRLAHHL RSLGVGPEST VALLLPRSPE QVTAVLAVLK AGGACLPVDP DYPAERIRHM
LADARPSRVL ALRSTADRLP EPAAALVLDD PLTARTLEAA PDTDPADQDR TAALLTAHPA
YVIHTSGSTG RPNGVVVTHE GIANLAARQI DRFQVTAADR VLQFASPSFD AAFSELCMAL
LSGAELVLAT REELMPGRPL AELIARHRVT HVTLPPVALS VLPPDGLATV RTLAVAGEAM
PADAVHRWGS GRRLINAYGP TETTVCATMS EPLTGQDRPS IGRPIGGAGV RVLDERLRPV
PPGVAGELYV SGPGLARGYL GRPGLTAQRF LPDPFGPPGS RMYRTGDLVR WDREGRLHFL
GRADHQLSVR GFRVEPGEIE AALLDHPAVA QAVLTVREDG RGEPRLIGYA VPHGDAPDPA
ELRAHAAGLL PAHMVPAAVI VLPALPVTPN GKLDRAALPE PDFSGQPDRA ARSPREAVLR
ELFAEVLGAP DLGVHDSFFD AGGHSMLAAR LIDRVHTVLG LDLGLRLLFE SPSVAELAAA
LDGAADGSRR GGDWDVLLPL RPGGGRAPVF CVHPAAGLGW SFAGLARYLD DCHPLYALQS
PGLEGGPDRL PATVADAAAA YVAEIRRVAP QGPYHLLGWS AGGLIAHEMA VQLQAAGEQV
GLLAVLDGYP LAGLAPVAAP SPEEVAEAVR RETAGLQLDD RVRDNLAAVY LGVARAAQAF
TPGVFNGELL HFAATHDRDR AHDRGQDRDH DRLEAKLWEP HLDGPLRTHE VACGHEEMTR
PQALGVIGPA LADRLRELTA PTQHTGGTDR
//
