ID A0A1Q4WKP0_9ACTN Unreviewed; 1142 AA.
AC A0A1Q4WKP0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AMK13_05540 {ECO:0000313|EMBL:OKI10557.1};
OS Streptomyces sp. CB02056.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703924 {ECO:0000313|EMBL:OKI10557.1, ECO:0000313|Proteomes:UP000186379};
RN [1] {ECO:0000313|EMBL:OKI10557.1, ECO:0000313|Proteomes:UP000186379}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB02056 {ECO:0000313|EMBL:OKI10557.1,
RC ECO:0000313|Proteomes:UP000186379};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKI10557.1}.
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DR EMBL; LIPD01000002; OKI10557.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q4WKP0; -.
DR OrthoDB; 5240387at2; -.
DR Proteomes; UP000186379; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF59; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000186379}.
FT DOMAIN 31..333
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 341..687
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 512..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 52..59
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1142 AA; 124181 MW; 84793891DF544831 CRC64;
MPRHAPVRPQ DPFVTSPYRL VRSPLVQPDP PVLDAYQRAV VEHPGGPLLV LAGPGTGKTT
TLVEAVARRI EDGTDPERIL VLTFSRKAAT ELRDRMSGRL GGTAAPQATT FHSYCYALLR
AHQDPEDYAE PLRLLSGPEQ DVMVRELLAG GAEDAKLGLG RIAWPLDLRA CLTTRGFADE
VRAVLARSRE LGLGEAELER FAQGVQRPDW AAAAHFLADY LDVLDLRGVL DYAELVHRAV
LLAERPDVAE QLRRRYDVVF VDEYQDTDPS QVRLLRRLAG GGRDLVAVGD PDQSIYAFRG
ADINGILDFP QTFRQADGSP AEVKVLRVSR RSGALLLAAS REVARRMPMG RLPADKLAQH
RALLPSREGG RVEVYTYPTP GAELDSIADL LRRAHLEDGV PWGEMAVLVR AGARSIPGVR
RALGAAGVPL EIDGDDLPLR EEPAVAQLLL ALRVCAEQAA RDKQGDPDAP DPLTTDLAHA
LLTGPLGGLD GSDLRRLGRA LREEERAALR AEARARLRTP EGGTDDGSAT TDPGPGPGPA
AAPRPAGELI REALAEPERL VALDPAHARR ARDLGTLLRK VRELLAGGCT AEEALWELWD
GSRRWRERLE RAALRGGAAG RNADRDLDAL CALFETAARA EEQVTGHRSA LDLLAELEAQ
DIAADTLTVR TVRPEAVRLM TAHRSKGLEW RLVVVAGVQD GLWPDLRRRG SLLEADRIGR
DGLAEPLSPG ALLGEERRLF YVAATRAKER LIVTAVKAPA DDGDEPSRFL RELYREDVDP
RTGRVVRRTP PVTVEDVTHR PRRPLSVAAL VAELRAVTVD PARSPELRRA AAERLATLAA
ATDEEGQPLV PAARPERWWG LDDPTAAAEP LRSADAPVRL SGSGLEQLES CSLQWFLDKE
VKARGAGSAA QGFGNVVHAL ADEVGSGRTP ADLAVLMERL DTVWDALAFD APWKSQQEKT
EARAALERFL NWHVLERGRD LVATEHGFDV TLDVGGVSVR IRGSMDRVEK DSAGRAYVVD
FKTGKQIPTD RSLPEHKQLA VYQLAVRAGA LNELPGFPGH PPDTGGAELV HLREPAAKAA
QDTPKVQHQE PPQGEPWIEN LLAEAAGKVL AERFVPQTGG GCDRCTFRRS CSAQRDGAQL
VE
//
