ID A0A1Q4WZE8_9PSEU Unreviewed; 446 AA.
AC A0A1Q4WZE8;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=L-lysine N6-monooxygenase MbtG {ECO:0000256|ARBA:ARBA00016406};
DE EC=1.14.13.59 {ECO:0000256|ARBA:ARBA00013076};
DE AltName: Full=Lysine 6-N-hydroxylase {ECO:0000256|ARBA:ARBA00032738};
DE AltName: Full=Lysine N6-hydroxylase {ECO:0000256|ARBA:ARBA00032493};
DE AltName: Full=Lysine-N-oxygenase {ECO:0000256|ARBA:ARBA00029939};
DE AltName: Full=Mycobactin synthase protein G {ECO:0000256|ARBA:ARBA00031158};
GN ORFNames=A6A25_14000 {ECO:0000313|EMBL:OKI15422.1};
OS Saccharothrix sp. CB00851.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharothrix.
OX NCBI_TaxID=1835005 {ECO:0000313|EMBL:OKI15422.1, ECO:0000313|Proteomes:UP000186828};
RN [1] {ECO:0000313|EMBL:OKI15422.1, ECO:0000313|Proteomes:UP000186828}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB00851 {ECO:0000313|EMBL:OKI15422.1,
RC ECO:0000313|Proteomes:UP000186828};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysine + NADPH + O2 = H2O + N(6)-hydroxy-L-lysine + NADP(+);
CC Xref=Rhea:RHEA:23228, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32551, ChEBI:CHEBI:57783, ChEBI:CHEBI:57820,
CC ChEBI:CHEBI:58349; EC=1.14.13.59;
CC Evidence={ECO:0000256|ARBA:ARBA00000168};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-
CC oxygenase family. {ECO:0000256|ARBA:ARBA00007588}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKI15422.1}.
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DR EMBL; LWLC01000064; OKI15422.1; -; Genomic_DNA.
DR RefSeq; WP_073899150.1; NZ_LWLC01000064.1.
DR AlphaFoldDB; A0A1Q4WZE8; -.
DR STRING; 1835005.A6A25_14000; -.
DR Proteomes; UP000186828; Unassembled WGS sequence.
DR GO; GO:0047091; F:L-lysine 6-monooxygenase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR025700; Lys/Orn_oxygenase.
DR PANTHER; PTHR42802:SF1; L-ORNITHINE N(5)-MONOOXYGENASE; 1.
DR PANTHER; PTHR42802; MONOOXYGENASE; 1.
DR Pfam; PF13434; Lys_Orn_oxgnase; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:OKI15422.1};
KW Oxidoreductase {ECO:0000313|EMBL:OKI15422.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000186828}.
SQ SEQUENCE 446 AA; 49409 MW; 3B5D54726C200D07 CRC64;
MPQPPVFDVL GVGFGPSNLA LAIAVTEYNA AARPEDVVTA HFLERQPRFG WHRGMLIEDA
TMQVSFLKDL VTLRNPNSTF TFLSYLHNKG RLVDFINHKN LFPLRVEFHD YFEWAAGRVD
DLVSYDHDVV DVRPVTEDGV ITHFDVVSRT SAGEVEVRRA RNLVLATGLR PALPEGVRPS
DRVWHNRDFL HRVDSLTDPR RLVVVGAGQS GAEVTAFLHE RFPSAEVCAV FARYGYSPAD
DSSFANRIFD PAAVDDYFAA PEQVKRKLMD YHGNTNYAVV DNELIAELYR RVYREKVVGR
QRLRLLNLSR VAELDDRADG VAVTVESLGT GAREVLDADA IVYATGYREA DPTALLGELA
GHCARDAHGR LSVGRDYRLD TDDDLPAGLY LQGGTEHTHG ISSSLLSNTS VRVGEILRSV
LGRRTPAVPS GVPSQPEYAI SVSRVS
//
