ID A0A1Q5GL17_9ACTN Unreviewed; 358 AA.
AC A0A1Q5GL17;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=AMK22_21270 {ECO:0000313|EMBL:OKJ33304.1};
OS Streptomyces sp. CB01580.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703933 {ECO:0000313|EMBL:OKJ33304.1, ECO:0000313|Proteomes:UP000186318};
RN [1] {ECO:0000313|EMBL:OKJ33304.1, ECO:0000313|Proteomes:UP000186318}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB01580 {ECO:0000313|EMBL:OKJ33304.1,
RC ECO:0000313|Proteomes:UP000186318};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKJ33304.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LIXJ01000015; OKJ33304.1; -; Genomic_DNA.
DR RefSeq; WP_073787486.1; NZ_LIXJ01000015.1.
DR AlphaFoldDB; A0A1Q5GL17; -.
DR STRING; 1703933.AMK22_21270; -.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000186318; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR CDD; cd04730; NPD_like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Dioxygenase {ECO:0000313|EMBL:OKJ33304.1};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000186318}.
FT DOMAIN 3..344
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 358 AA; 36974 MW; 8956A47EFAC34A99 CRC64;
MSSVLTDLCR YPIVQAPMAG GTSRPELAAA VSEAGGLGFL AAGYKTADGM YNEIKRLRTL
TGRPFGVNLF MPQPDLADPS AVDVYRHQLA GEGAWYETPL GDPDAGGGDD GYEAKLAVLL
EDPVPAVSFA FGCPSRDTLD ALANVGTYTV VTVTTPEEAQ AAQWAGADAV CAQGVEAGGH
QSTHRDDPLN DGTGIGLLSL VAQVRETVEM PIVAAGGLMR GSQIAAVLAA GAQAAQLGTA
FLVCPESGAN PLHKQALTNP LFAGTALTRA FSGRPARGLI NRFMREHGPY APAAYPQIHY
LTAGLRKAAA TSGDAQGMAL WAGQGHRMAR EVPAGRLVGL LVEELDAARA ALNAGGTR
//
