ID A0A1Q5HK58_9ACTN Unreviewed; 109 AA.
AC A0A1Q5HK58;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Hydrogenase maturation factor HypA {ECO:0000256|HAMAP-Rule:MF_00213};
GN Name=hypA {ECO:0000256|HAMAP-Rule:MF_00213};
GN ORFNames=AMK22_00925 {ECO:0000313|EMBL:OKJ45190.1};
OS Streptomyces sp. CB01580.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703933 {ECO:0000313|EMBL:OKJ45190.1, ECO:0000313|Proteomes:UP000186318};
RN [1] {ECO:0000313|EMBL:OKJ45190.1, ECO:0000313|Proteomes:UP000186318}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB01580 {ECO:0000313|EMBL:OKJ45190.1,
RC ECO:0000313|Proteomes:UP000186318};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC for nickel insertion into the metal center of the hydrogenase.
CC {ECO:0000256|HAMAP-Rule:MF_00213}.
CC -!- SIMILARITY: Belongs to the HypA/HybF family.
CC {ECO:0000256|ARBA:ARBA00010748, ECO:0000256|HAMAP-Rule:MF_00213}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKJ45190.1}.
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DR EMBL; LIXJ01000001; OKJ45190.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q5HK58; -.
DR STRING; 1703933.AMK22_00925; -.
DR OrthoDB; 288014at2; -.
DR Proteomes; UP000186318; Unassembled WGS sequence.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR GO; GO:0036211; P:protein modification process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.2320.80; -; 1.
DR HAMAP; MF_00213; HypA_HybF; 1.
DR InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR InterPro; IPR000688; HypA/HybF.
DR PANTHER; PTHR34535; HYDROGENASE MATURATION FACTOR HYPA; 1.
DR PANTHER; PTHR34535:SF3; HYDROGENASE MATURATION FACTOR HYPA; 1.
DR Pfam; PF01155; HypA; 1.
DR PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR PROSITE; PS01249; HYPA; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_00213};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_00213};
KW Reference proteome {ECO:0000313|Proteomes:UP000186318};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|HAMAP-Rule:MF_00213}.
FT BINDING 2
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00213"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00213"
FT BINDING 73
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00213"
FT BINDING 86
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00213"
FT BINDING 88
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00213"
SQ SEQUENCE 109 AA; 11738 MW; CE3345CA18B78E3C CRC64;
MHELSITQGI VEAVCERADG RPVRAVRVRV GILTAVVPEA MRFCFDLTTA GTAAEDALLE
IDHVPGRAHC RACETDFLLN DLVLLCPCGS SDVDVTSGRE LQIVSMRVG
//
