ID A0A1Q5J9E1_9ACTN Unreviewed; 340 AA.
AC A0A1Q5J9E1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:OKJ65969.1};
GN ORFNames=AMK29_14635 {ECO:0000313|EMBL:OKJ65969.1};
OS Streptomyces sp. CB02261.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703940 {ECO:0000313|EMBL:OKJ65969.1, ECO:0000313|Proteomes:UP000186742};
RN [1] {ECO:0000313|EMBL:OKJ65969.1, ECO:0000313|Proteomes:UP000186742}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB02261 {ECO:0000313|EMBL:OKJ65969.1,
RC ECO:0000313|Proteomes:UP000186742};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKJ65969.1}.
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DR EMBL; LIVX01000004; OKJ65969.1; -; Genomic_DNA.
DR RefSeq; WP_073812797.1; NZ_LIVX01000004.1.
DR AlphaFoldDB; A0A1Q5J9E1; -.
DR OrthoDB; 9782387at2; -.
DR Proteomes; UP000186742; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR022563; DUF3463.
DR InterPro; IPR017833; Hopanoid_synth-assoc_rSAM_HpnH.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR03470; HpnH; 1.
DR PANTHER; PTHR11228:SF22; PEPTIDE BIOSYNTHESIS PROTEIN YYDG-RELATED; 1.
DR PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR Pfam; PF11946; DUF3463; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00397; adenosyl-hopene_transferase; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 24..231
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 340 AA; 38539 MW; 8DC4B55D188E394F CRC64;
MAMPLRQSIR VGTYLFEQKL RKREKFPLIV ELEPLFACNL KCEGCGKIQH PAGVLKQRMP
VAQAVGAVLE SGAPMVSIAG GEPLMHPQIH EIVRQLVAKR KYVFLCTNAM LLRKKLENFT
PSPYFAFAVH IDGLRERHDE SVAKEGVFDE AVAAMKEAKR RGFRVTTNST FFNTDTPQTI
IEVLNFLNDD LQVDEMMISP AYAYEKAPDQ EHFLGVEQTR ELFKKAFAGG NRRRWRLNHS
PLFLDFLEGK ADFPCTAWAI PNYSLFGWQR PCYLMSDGYV PTYRELVEDT DWDKYGRGKD
PRCANCMAHC GYEPTAVLAT MGSLKESLRA VRETVSGNNG
//
