ID A0A1Q5L5H8_9ACTN Unreviewed; 587 AA.
AC A0A1Q5L5H8;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=FAD-binding dehydrogenase {ECO:0000313|EMBL:OKJ89202.1};
GN ORFNames=AMK31_04395 {ECO:0000313|EMBL:OKJ89202.1};
OS Streptomyces sp. TSRI0107.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703942 {ECO:0000313|EMBL:OKJ89202.1, ECO:0000313|Proteomes:UP000185813};
RN [1] {ECO:0000313|EMBL:OKJ89202.1, ECO:0000313|Proteomes:UP000185813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TSRI0107 {ECO:0000313|EMBL:OKJ89202.1,
RC ECO:0000313|Proteomes:UP000185813};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKJ89202.1}.
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DR EMBL; LIVZ01000002; OKJ89202.1; -; Genomic_DNA.
DR RefSeq; WP_073939047.1; NZ_LIVZ01000002.1.
DR AlphaFoldDB; A0A1Q5L5H8; -.
DR STRING; 1703942.AMK31_04395; -.
DR Proteomes; UP000185813; Unassembled WGS sequence.
DR GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.90.700.10; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR InterPro; IPR003953; FAD-binding_2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR014614; KsdD_DH.
DR InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR InterPro; IPR006311; TAT_signal.
DR PANTHER; PTHR43260; 3-KETOSTEROID-DELTA-1-DEHYDROGENASE; 1.
DR PANTHER; PTHR43260:SF1; KSDD-LIKE STEROID DEHYDROGENASE RV0785; 1.
DR Pfam; PF00890; FAD_binding_2; 1.
DR PIRSF; PIRSF036654; UCP036654; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS51318; TAT; 1.
PE 4: Predicted;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000185813};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..587
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013135353"
FT DOMAIN 36..567
FT /note="FAD-dependent oxidoreductase 2 FAD binding"
FT /evidence="ECO:0000259|Pfam:PF00890"
SQ SEQUENCE 587 AA; 62967 MW; D3AA328FCED70272 CRC64;
MPGITRRHAL TAAGGVLAAT LAGAMPAFAA ESQDADAIVV GHGLAGLVAT AELAAAGRKV
LLLDQEPEAS LGGQAFWSFG GLFLVDSDEQ RLMGIKDSHD LAWQDWLGTA GFDRGVDDPS
GQDHWGRKWA QAYVEFASGE KRSWLAGLGM QWFPVVGWAE RGGGLADGHG NSVPRFHVTW
GTGPAVVEPF EKKVRAARAD GKVSFRFRHR VDGIVTTGGA VTGVRGAILE PSDAARGRPS
SRVQVGEFEL RAPVVVVTSG GIGADHDLVR RNWPARLGTP PRTMVTGVPA HVDGRMLAIT
ERAGGRIVNP DRMWHYTEGL RNYDPIWPGH GIRILPGPSS MWFDATGKRF GSPDIPGYDT
LHTLKSITGT GYDYSWFVTT QKILAKEFAL SGSEQNPDLT EKNVWMLLSR IWQTPEPIER
FKKKGADFVV ATTLPELVSG MNRLTGDNLI RLDDLRRQIE ARDREIDNPY TKDVQVMGIR
NALSYVGDSL SRTAPAHRIL DSAAGPLIAV RLNILTRKTL GGLQTDLSGR VLNASGDPVP
GLYAAGEVAG FGGGGVHGYR SLEGTFLGGC LFSGRQTGRA AAQATAT
//
