ID A0A1Q5NDE0_9ACTN Unreviewed; 570 AA.
AC A0A1Q5NDE0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=3'-5' exonuclease {ECO:0000313|EMBL:OKK24477.1};
GN ORFNames=AMK09_06700 {ECO:0000313|EMBL:OKK24477.1};
OS Streptomyces sp. CB02488.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1703920 {ECO:0000313|EMBL:OKK24477.1, ECO:0000313|Proteomes:UP000186200};
RN [1] {ECO:0000313|EMBL:OKK24477.1, ECO:0000313|Proteomes:UP000186200}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CB02488 {ECO:0000313|EMBL:OKK24477.1,
RC ECO:0000313|Proteomes:UP000186200};
RX PubMed=27999165; DOI=10.1128/mbio.02104-16;
RA Yan X., Ge H., Huang T., Hindra, Yang D., Teng Q., Crnovcic I., Li X.,
RA Rudolf J.D., Lohman J.R., Gansemans Y., Zhu X., Huang Y., Zhao L.X.,
RA Jiang Y., Van Nieuwerburgh F., Rader C., Duan Y., Shen B.;
RT "Strain Prioritization and Genome Mining for Enediyne Natural Products.";
RL MBio 7:e02104-e02116(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKK24477.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LIVO01000002; OKK24477.1; -; Genomic_DNA.
DR RefSeq; WP_073733639.1; NZ_LIVO01000002.1.
DR AlphaFoldDB; A0A1Q5NDE0; -.
DR STRING; 1703920.AMK09_06700; -.
DR OrthoDB; 4414061at2; -.
DR Proteomes; UP000186200; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd06444; DNA_pol_A; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002298; DNA_polymerase_A.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 3: Inferred from homology;
KW Exonuclease {ECO:0000313|EMBL:OKK24477.1};
KW Hydrolase {ECO:0000313|EMBL:OKK24477.1};
KW Nuclease {ECO:0000313|EMBL:OKK24477.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000186200}.
FT DOMAIN 311..529
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 104..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 426..469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 570 AA; 61685 MW; EAA692B2F63E57B6 CRC64;
MTERWAVAAT ESGGARLVPL DAAGLPTAPA VTEPELAEAV RSRPGVTRWV WRSTAEIYPR
LLAAGVSVAR CYDIEAAESL LLGHEGRLGE PRSAAAAHAR LLRAPVPPDP APRQAVPGSQ
SSLFEPRSGT ELPFDALLQV YADQLARHDA TARPDRMRLL TASESAGMLV AAEMNRAGLP
WRADVHRDVL QELLGERYAG GEPRRLAELA DEVSAAFGHR VRPDLPADVV RAFGRAGIKV
KSTRRWELEE LDHPAVRPLI QYKKLYRIWT AHGWSWLQDW VRDGRFRPEY QPGGTVSGRW
TTNGGGALQI PKVIRRAVVA DEGWRLVVAD ADQMEPRVLA AISRDRGLME VAGHDGDLYT
ALSDRAFHGD RDHAKIALLG AVYGQTSGDG LKNLAALRRR FPHAVAYVDE AAKAGEEGRL
VRTWLGRTSP PAAGSGDDGE AGIPQEGGDD LPGEGSGSPA DGGFTPGYAS SDARARGRFT
RNFVVQGSAA DWALLLLAAL RHSLTAAGLR AELVFFQHDE VIVHCPREEA ETVVEAIRAA
GELAGRTTFG ETPVRFPFTT AVVRRYSDAK
//
