ID   A0A1Q5PJC4_9ACTO        Unreviewed;       701 AA.
AC   A0A1Q5PJC4;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=formate C-acetyltransferase {ECO:0000256|ARBA:ARBA00013214};
DE            EC=2.3.1.54 {ECO:0000256|ARBA:ARBA00013214};
GN   ORFNames=BSR29_08405 {ECO:0000313|EMBL:OKL45991.1};
OS   Boudabousia liubingyangii.
OC   Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC   Boudabousia.
OX   NCBI_TaxID=1921764 {ECO:0000313|EMBL:OKL45991.1, ECO:0000313|Proteomes:UP000186785};
RN   [1] {ECO:0000313|EMBL:OKL45991.1, ECO:0000313|Proteomes:UP000186785}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VUL4_2 {ECO:0000313|EMBL:OKL45991.1,
RC   ECO:0000313|Proteomes:UP000186785};
RA   Meng X.;
RT   "Actinomyces gypaetusis sp. nov. isolated from the vulture Gypaetus
RT   barbatus in Qinghai Tibet Plateau China.";
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + formate = CoA + pyruvate; Xref=Rhea:RHEA:11844,
CC         ChEBI:CHEBI:15361, ChEBI:CHEBI:15740, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.54;
CC         Evidence={ECO:0000256|ARBA:ARBA00001179};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family. PFL
CC       subfamily. {ECO:0000256|ARBA:ARBA00008375}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00493}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OKL45991.1}.
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DR   EMBL; MQSV01000007; OKL45991.1; -; Genomic_DNA.
DR   RefSeq; WP_073709858.1; NZ_MQSU01000002.1.
DR   AlphaFoldDB; A0A1Q5PJC4; -.
DR   STRING; 1921764.BSR28_04420; -.
DR   OrthoDB; 9803969at2; -.
DR   Proteomes; UP000186785; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.70.20; -; 1.
DR   InterPro; IPR001150; Gly_radical.
DR   InterPro; IPR004184; PFL_dom.
DR   PANTHER; PTHR30191; FORMATE ACETYLTRANSFERASE; 1.
DR   PANTHER; PTHR30191:SF0; FORMATE ACETYLTRANSFERASE 1; 1.
DR   Pfam; PF01228; Gly_radical; 1.
DR   Pfam; PF02901; PFL-like; 1.
DR   PIRSF; PIRSF000379; For_Ac_trans_1; 1.
DR   SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR   PROSITE; PS51149; GLY_RADICAL_2; 1.
DR   PROSITE; PS51554; PFL; 1.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Organic radical {ECO:0000256|ARBA:ARBA00022818};
KW   Reference proteome {ECO:0000313|Proteomes:UP000186785};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OKL45991.1}.
FT   DOMAIN          10..628
FT                   /note="PFL"
FT                   /evidence="ECO:0000259|PROSITE:PS51554"
FT   DOMAIN          635..701
FT                   /note="Glycine radical"
FT                   /evidence="ECO:0000259|PROSITE:PS51149"
FT   ACT_SITE        421
FT                   /note="S-acetylcysteine intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
FT   ACT_SITE        422
FT                   /note="Cysteine radical intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000379-1"
SQ   SEQUENCE   701 AA;  77895 MW;  16B65937366003DB CRC64;
     MAKDDTAQVE ESAALPEAWE GFVPGNWCEN IDVRDFIQLN YTPYEGDASF LSGPTEKTLR
     TWETLEKEYL SVERAKRVYD VDTKTPADVD AFGAGYISED DDVIVGLQTD VPLKRAMMPN
     GGWRMVETAI REAGKEPDPE IKKIFTQYRK THNDAVFDIY TPRIRAARSS HIITGLPDAY
     GRGRIIGDYR RVALYGVDKL IEEKQKDKDL VADKPFSEHW CRYREEHAEQ IKALKKLKNM
     AASYGFDISG PATNAKEAVQ WTYFGYLASV KSQDGAAMSI GRLSGFFDSY FERDFKRGIL
     TEDEAQEIID ALVIKLRIVR FLRTIDYDAI FSGDPYWATW SDGGMGQDGR TLVTKTSFRL
     LQTLRNLGPA PEPNITLFWN KALPQGYKDF CALISIETSS VQYEADEQIR EHWGDDAAIA
     CCVSPMQVGK QMQFFGARVN AAKALLYAIN GGRDEMSGKQ VVNGYEPVQG DGPLDFEEVW
     AKYEDMLDWV VGTYVEALNI IHFCHDRYAY ESIEMALHDS EIVRTMGCGI AGLSIVADSL
     SAIRYAKVTP IRDETGLVVD YKTEGDFPVY GNDDDRADDI AATVVHTIMS KIKAIPMYRD
     AIPTQSVLTI TSNVVYGKAT GSFPSGHKAG TPFAPGANPE NGMDTHGMLA SMMSVGKLDY
     DDALDGISLT NTITPSGLGR TLDEQIKNLV GIMDAGFIPQ A
//