ID A0A1Q5PY22_9ACTO Unreviewed; 894 AA.
AC A0A1Q5PY22;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=BSZ40_02255 {ECO:0000313|EMBL:OKL52504.1};
OS Buchananella hordeovulneris.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Buchananella.
OX NCBI_TaxID=52770 {ECO:0000313|EMBL:OKL52504.1, ECO:0000313|Proteomes:UP000185612};
RN [1] {ECO:0000313|Proteomes:UP000185612}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20732 {ECO:0000313|Proteomes:UP000185612};
RA Meng X.;
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OKL52504.1}.
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DR EMBL; MQVS01000002; OKL52504.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q5PY22; -.
DR STRING; 52770.BSZ40_02255; -.
DR InParanoid; A0A1Q5PY22; -.
DR Proteomes; UP000185612; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000185612};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 14..274
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 650..857
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 894 AA; 97505 MW; 3CA944FBF6C6E8E5 CRC64;
MGSHGPRVAQ VSTSRLLLID GHSMAFRAYY ALPVDNFTTS AGQATNAVHG FTSMLLRLLE
AEKPTHVAVA FDTKAPTFRL QQYPEYKGTR EATPEPFKGQ VSLIQDVLDA VGVAHFSLDG
FEADDILATL ATQGAAAGAE VLLCSGDRDT FQLVTEHVTV LYPVKGVSEL RRMTPAEVEA
RYGVPPQRYP HLAALVGETS DNLPGVEGVG PKTAADWLRK YDGLEGVLAH ADQITGKRGQ
ALRDSVEQVR RNRQLNRLLT DVELGVELAD LRPRGVDAAA LHETFDALEF ATLRERVLAQ
LDVADGATAV VDAAPTELVV VDATVAPERA AAQLEAWQRD GTLVGMDVEG VASPDPARAD
AWEVAFTDGQ TAVVYDLQEE PVAVQIFCAS LSQLAVCDLK AIMHMLNGRL VAIGAPVFDV
ALAAYLCHPD GRKFDVAELT RRYLGRELEA DSDTLDLDGT SRPTVMRAQA VRQLADVLAA
EVERRSARQL LDDVELPVSR CLWAMENWGI AVDRERLAQL HAEFDAEVQA AARGAYDAIG
KEINLSSPKQ LQQVLFTDLA LPKTRKTKTG YSTDAEALTD LAVAHPHPFL DHLLAHRDKI
KLRQIVETLQ KSVSDDGRIH STFQQTVAAT GRLSSTDPNL QNIPARTPEG RAIRSAFVAG
DGGECLLTAD YSQIEMRILA HLTEDPGLIE AFHSGEDLHS YTASRVFGGT PDAVTPQQRS
RIKAMTYGLA YGLSAYGLSK QLRIEVAEAN RLRDDFFARF GAVRDYLEQV VAQARVRGYT
ETIMGRRRYL PDLTSDNRQR REMAERAALN APIQGSAADI IKVAMLNTVR ALFEAELESS
ILLQVHDELV LEIAPGELEA VQEIVRREMS GAVSLRVPLD VSMGSGTNWL EAGH
//
