ID   A0A1Q5Q1I4_9ACTO        Unreviewed;      1085 AA.
AC   A0A1Q5Q1I4;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   24-JAN-2024, entry version 21.
DE   RecName: Full=HNH Cas9-type domain-containing protein {ECO:0000259|PROSITE:PS51749};
GN   ORFNames=BSZ39_08295 {ECO:0000313|EMBL:OKL53677.1};
OS   Bowdeniella nasicola.
OC   Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC   Bowdeniella.
OX   NCBI_TaxID=208480 {ECO:0000313|EMBL:OKL53677.1, ECO:0000313|Proteomes:UP000185628};
RN   [1] {ECO:0000313|Proteomes:UP000185628}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 19116 {ECO:0000313|Proteomes:UP000185628};
RA   Meng X.;
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OKL53677.1}.
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DR   EMBL; MQVR01000046; OKL53677.1; -; Genomic_DNA.
DR   RefSeq; WP_073716877.1; NZ_MQVR01000046.1.
DR   AlphaFoldDB; A0A1Q5Q1I4; -.
DR   OrthoDB; 9802901at2; -.
DR   Proteomes; UP000185628; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.3520; -; 1.
DR   Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 3.
DR   InterPro; IPR040619; Cas9_alpha-helical_lobe.
DR   InterPro; IPR040796; Cas9_b_hairpin.
DR   InterPro; IPR041217; Cas9_C.
DR   InterPro; IPR041225; Cas9_Topo.
DR   InterPro; IPR002711; HNH.
DR   InterPro; IPR033114; HNH_CAS9.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR041383; RuvC_III.
DR   Pfam; PF18470; Cas9_a; 1.
DR   Pfam; PF17893; Cas9_b_hairpin; 1.
DR   Pfam; PF18525; Cas9_C; 1.
DR   Pfam; PF17894; Cas9_Topo; 1.
DR   Pfam; PF01844; HNH; 1.
DR   Pfam; PF18541; RuvC_III; 1.
DR   SMART; SM00507; HNHc; 1.
DR   PROSITE; PS51749; HNH_CAS9; 1.
PE   4: Predicted;
KW   Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PROSITE-
KW   ProRule:PRU01085}; Endonuclease {ECO:0000256|PROSITE-ProRule:PRU01085};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01085}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nuclease {ECO:0000256|PROSITE-ProRule:PRU01085};
KW   Reference proteome {ECO:0000313|Proteomes:UP000185628};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT   DOMAIN          506..668
FT                   /note="HNH Cas9-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51749"
FT   REGION          338..358
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1085 AA;  121772 MW;  DB284616FB53D25E CRC64;
     MADLLPYVVG IDVGTHSIGA VAIEVDEKNN PISILNAVVH IHDSGVDPDK RKTAESRLKT
     AGVARRTRRL TRRRRRRLQQ LDALLEHLGY PFPDLAERPD PHTPWKVRAR LVRETIADEH
     ERKTAISIAL RHIARHRGWR NPYSRVESLL HPAEDSQQLL DLKERVKEIK PELDLAAATS
     AEVITSVDMY NVKFRGPEGM LGGKLMQSDN ANEIRRICEM QGVPDDEARR LIQAVFKAES
     PRGSSAERIG FDALPGQTHM HRAAKAHPSF QLFRMLSILT NLRIREGKGQ TRRLSVDELQ
     LAVGHLTTLK PKVVPIWADV AEVLGIERSQ LVGTATPTVD GERAAARPPV DETSRRMQAH
     APKSVKAWWV EASDEEQAGF IEQVTDGIPS ETPAVRDFFD SLDDDDLVAL DSLTLTGGRA
     AYSVNSLDRL SERMLHTGED LHEARKSVFG VDDDWAPPVP SIGEPVGNPA VDRVLRQIAR
     WLLAVEKRWG EPKSINIEHV RDGFSSEAVS RELDRQYNKR REINERYAND LKERLNLSGP
     VTRSDIIRYS AVERQNSACA YCGREISFFN VELDHIVPRR GLGSTNRRDN LVAVCERCNR
     SKKNIPFVTW AAKADIPGVS LEEAISRVKF WVFPPGQMNA QQISSFKREV IARLKQRDFD
     PPLDGRSMES VAWMANELHG RVKAHFAGAS VRVFRGSITA AARKASGLER QIRFFGERGK
     TRLDRRHHAF DAACVAMMRP KVAEILTIRS NLRESQRLSR ETETWKNYSG ADAAEQFIYA
     NWLEQMQAMG DLFNDALDKD QVVVRENLRL RLANGAAHDA TIRKLQRKFL GEEFSVNEID
     KAATPALWCA LTRHPDFDNK NGLVANPDRV IRVNGTTLRA GDDVELFAQN SASIKVRGGS
     AEVGNSIHHA RVYRIDKGSR APAYGMVRVF QQDLLAHRHD DLFTTELKPQ SLSFRYADAT
     VKRAISEGRA TYLGWIVVGD EIRLDASQVA AGKIAEFMKE YPTIRSWRLD GFFSNSKLRL
     RPILLAAEGL SENASEASKA IIDRPGWVPA VNVVFGQLGA QIVRRTTLGE LRTREVSGMP
     ISWSV
//