ID A0A1Q7WEV3_9ACTN Unreviewed; 690 AA.
AC A0A1Q7WEV3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=UDP-glucose 4-epimerase {ECO:0000256|ARBA:ARBA00018569};
DE EC=5.1.3.2 {ECO:0000256|ARBA:ARBA00013189};
DE AltName: Full=Galactowaldenase {ECO:0000256|ARBA:ARBA00033067};
DE AltName: Full=UDP-galactose 4-epimerase {ECO:0000256|ARBA:ARBA00031367};
GN ORFNames=AUG49_00015 {ECO:0000313|EMBL:OLE29270.1};
OS Catenulispora sp. 13_1_20CM_3_70_7.
OC Bacteria; Actinomycetota; Actinomycetes; Catenulisporales;
OC Catenulisporaceae; Catenulispora.
OX NCBI_TaxID=1805055 {ECO:0000313|EMBL:OLE29270.1, ECO:0000313|Proteomes:UP000186605};
RN [1] {ECO:0000313|EMBL:OLE29270.1, ECO:0000313|Proteomes:UP000186605}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27843720;
RA Butterfield C.N., Li Z., Andeer P.F., Spaulding S., Thomas B.C., Singh A.,
RA Hettich R.L., Suttle K.B., Probst A.J., Tringe S.G., Northen T., Pan C.,
RA Banfield J.F.;
RT "Proteogenomic analyses indicate bacterial methylotrophy and archaeal
RT heterotrophy are prevalent below the grass root zone.";
RL PeerJ 4:E2687-E2687(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC EC=5.1.3.2; Evidence={ECO:0000256|ARBA:ARBA00000083};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC {ECO:0000256|ARBA:ARBA00004947}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000256|ARBA:ARBA00007637}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLE29270.1}.
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DR EMBL; MNJN01000001; OLE29270.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q7WEV3; -.
DR UniPathway; UPA00214; -.
DR Proteomes; UP000186605; Unassembled WGS sequence.
DR GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR001509; Epimerase_deHydtase.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR005886; UDP_G4E.
DR NCBIfam; TIGR01179; galE; 1.
DR PANTHER; PTHR43725; UDP-GLUCOSE 4-EPIMERASE; 1.
DR PANTHER; PTHR43725:SF51; UDP-GLUCOSE 4-EPIMERASE; 1.
DR Pfam; PF01370; Epimerase; 1.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023144};
KW Galactose metabolism {ECO:0000256|ARBA:ARBA00023144}.
FT DOMAIN 13..259
FT /note="NAD-dependent epimerase/dehydratase"
FT /evidence="ECO:0000259|Pfam:PF01370"
FT DOMAIN 391..498
FT /note="Glycosyltransferase 2-like"
FT /evidence="ECO:0000259|Pfam:PF00535"
FT REGION 351..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..384
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 690 AA; 75309 MW; 5B93A4C8A91D82B0 CRC64;
MSDPAASEEI RKVLITGGAG FIGSTVASAC LDAGLTPVIL DNLSTGRREF TAGRIFYEGD
IADAALLDRI FADHPDLAVT VHCAALTIVP ESVANPIRYY RENVTKTLEL IEGLVRNDCR
RLVFSSSASV YAAGAGGEAG EVDEDAPIAP SNPYARTKAI TEWVLEDVAR TGELRAVALR
YFNPIGADPE FRTGNPNPEP SHVLGKMITA YQGAEPFHIT GVDWPTRDGS AIRDYIHVWD
LAEAHVAALR NFDAIVARHI VPETSRSEVP YEVINLGTGD GTTVRELAEA FQDVVPGELD
VRTAPRRPGD VIGAYTGTAK ARALLGWAPK HTVAEGIRDA LIWARTLAGT SAPSRSADTD
TDADTDTAAA RQDRSRRSDA KAKPPEPVVD VLMPYYGDVG LMQEAVRSVL AQRDPHWRLT
VVDDGTETGV PEWFDELIAE HGTDKIHYRR NATNLGVTEN FQKCLTLVTC PLVTMIGCDD
RMLPNYIGTV RALMKAFPQV SLAQPGVEVI DDEGAVVEPW VDKVKRRVYA PRVQGAAVLG
GEELAVSLLR GNWMYFPAIC WRSDVITEVG FDPNLRVVQD LALTLEWVRA GAQIVVSDTV
CFQYRRHADS VSSERAYTGT RFTEERDFFL DEAAKMDRLG WRRAARAARL HLSSRLHAAT
LLPGALRRGS GAGARRLAGY AFGPTRRRIG
//
