ID A0A1Q8CNY9_9PSEU Unreviewed; 990 AA.
AC A0A1Q8CNY9;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Multifunctional fusion protein {ECO:0000256|HAMAP-Rule:MF_00254, ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE AltName: Full=Glycyl-tRNA synthetase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
GN Name=glyQ {ECO:0000256|HAMAP-Rule:MF_00254};
GN Synonyms=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=BU204_19075 {ECO:0000313|EMBL:OLF16048.1};
OS Actinophytocola xanthii.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae.
OX NCBI_TaxID=1912961 {ECO:0000313|EMBL:OLF16048.1, ECO:0000313|Proteomes:UP000185596};
RN [1] {ECO:0000313|EMBL:OLF16048.1, ECO:0000313|Proteomes:UP000185596}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=11-183 {ECO:0000313|EMBL:OLF16048.1,
RC ECO:0000313|Proteomes:UP000185596};
RA Wang W., Yuan L.;
RT "The draft genome sequence of Actinophytocola sp. 11-183.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLF16048.1}.
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DR EMBL; MSIE01000034; OLF16048.1; -; Genomic_DNA.
DR RefSeq; WP_075127100.1; NZ_MSIE01000034.1.
DR AlphaFoldDB; A0A1Q8CNY9; -.
DR STRING; 1912961.BU204_19075; -.
DR OrthoDB; 9775440at2; -.
DR Proteomes; UP000185596; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR NCBIfam; TIGR00388; glyQ; 1.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000185596}.
SQ SEQUENCE 990 AA; 106207 MW; 104273547359A138 CRC64;
MQDALLALTR YWTDRGCMIV QPFNTEVGAG TLNPATILRV LGPEPWRVAY VEPSVRPDDS
RYGENPNRLQ THTQFQVILK PDPGDPQEQY LASLEALGID IHAHDIRFVE DNWANPATGS
WGLGWEVWLD GLEITQFTYF QQAGGMSLDP VSVEITYGVE RIIMALQGVS HFKDIQYAPG
ISYGEAFGQA EYEMSRFYLD DADVDTQRRL FEDYAAEARR LLDARLPVPA HVHVLKCSHT
FNVLDARGAV STTERARAFA RMRALAREVS ALWAQRREEL GHPLGLTPAL SAAAPAESLP
EVSAAAPLLF EIGTEELPPG EVTRSARAVR EALTAKLAGT RLEHGAVEVH ATPRRIVVVV
EEVAPREPDA ERFVRGPRVS AAFDADGNPT KAAAGFARGQ GVEVSALERV EVDGVEHVGV
TRTDAGRGAV EVLAEVLGSL VAELRADKNM RWNDPKLSFT RPVRWLVALL GSEVVPVVAS
SLAAGRTTRV HRTAESPVVE VPSASGYLDF LASHGIVASP SARRATIVSA ATALAESVGG
RIDVEGEAAL LDEITNLVEE PNAILGGFAP SYLDLPQEIL TTVMRKHQRY VPVRDAEGNL
LPHFVAVANG SCAADAVRAG NEAVLRARYE DASFFWRADL QVAPEAMKSG LARLAFEERL
GSMADRAARI AAVAGNLGSA LSSADRATLD RAAVLAKFDL GSQMVIELTS LAGVMAREYA
VRAGETPEVA QALFDMERPR SAGDAAPATV PGAVLALADR FDMLVGLFAV GAAPTGSSDP
FGLRRAASGV ITILRAHPSL REITFERGLA AAAQHVRAQG IEVAEATLAE AAEFITRRQE
QQLLDAGHDH RHVAAVLALP PATAAETLAE IESRQGNEEF AALAAALQRV RRIVPADTAP
SYDTARLTEP AEVALHQALV KVADSLGAGP HSLAHFADVA SALTEPINTF FDEILVMAED
PEVRAARLGL LATIRDLAAP TLDWQALGTQ
//
