ID A0A1Q8CSI9_9PSEU Unreviewed; 971 AA.
AC A0A1Q8CSI9;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=BU204_11825 {ECO:0000313|EMBL:OLF17303.1};
OS Actinophytocola xanthii.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae.
OX NCBI_TaxID=1912961 {ECO:0000313|EMBL:OLF17303.1, ECO:0000313|Proteomes:UP000185596};
RN [1] {ECO:0000313|EMBL:OLF17303.1, ECO:0000313|Proteomes:UP000185596}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=11-183 {ECO:0000313|EMBL:OLF17303.1,
RC ECO:0000313|Proteomes:UP000185596};
RA Wang W., Yuan L.;
RT "The draft genome sequence of Actinophytocola sp. 11-183.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLF17303.1}.
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DR EMBL; MSIE01000018; OLF17303.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Q8CSI9; -.
DR STRING; 1912961.BU204_11825; -.
DR Proteomes; UP000185596; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000185596};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 23..137
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 149..475
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 634..661
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 971 AA; 108223 MW; 07C723FF66E2521D CRC64;
MQVDSLQYVT NDGFSRTLTA RLFDAFWAAG ISNPVETVEQ ISHLLYLREL DRLQEHWDQR
VAPSEMPEGG SIFAQGDQHL RWSHFLRLTP QRMYTSMADE VFPWLRSHTI AGVVYSQHVK
DARFTIPTPG LLAKTVSLLE ESFSAGDAAD LYEHLLAKAL TAGAMGQFLT PRHLAALMVA
MAEPGPDDEV CDPTCGMGGL LSAAAQFVDR SDPNTSQRSA LEVSSRLHGF DFDRTMLRLS
SMRLMLQGRE GADLRHRDNL VNRPGGDDER YSVVLADPPF GGNIDYKAVA PELLELVQTR
NSDLLHLAAI LRLLKRGGRA AVIVPAGLLF GTSAAHVELR RMLVDEHGLE AVVKLPNGAF
KPYSGVSGAI LFFIKDAGQA DSVWFYELKA DGWSLANRRA PLLAENKLGL SRDSTLDAGD
HARNNLPDLL RRWRLRHSNE RGRARTDQSF CVIRAEIAAE NYNLTLEHFR QTHELRQVAQ
EGIRLGDFAE TFSGAVRSSD LDKEPNSTDT DERRRVLTPT LLTSTLPDVA ELPVRADARD
PRHRLRQGDI VGRDLAGARH WTPIPSQYDG VQPGQGLIII RIIQEVLPLE YLIAYLSSPL
AEQQFPKYGT IPRIKAREMA DIWIPKCDGD PSEIRASLAR LEEGEREAAH IQDELRRART
RIFESGSGSA RRIRLDDAAA ISSLTAQNLR RHNDPYMLFQ ESYPYAVARA VRKFRHSLSL
AEKHEAAIQC TEALILSLGI MALAVAADRG RQDLPPIVQW SQSVEQGGVS LGHWLAVVKA
VAEDARQHGE PAVGLVEATA RKKGGTGLIA DLEQLVKLRN KIRHGAGPRT RAELEKSLGR
VETPMLSSLS GCAFLARTRW VHTERLQWLP TSGRFRVSGL ALMGDHPDFE MFTFDTSRPL
ANDHLYLITQ HDMPLPLSPF CLLSDCPTCL APELYYPDRM TRSTALLKSL DRGHELESEF
VFTTLQEWGR S
//
